Kazuko Sugai1, Hirokazu Kimura2, Yumiko Miyaji3, Hiroyuki Tsukagoshi4, Masakazu Yoshizumi4, Tomomi Sasaki-Sakamoto5, Satoko Matsunaga6, Yumi Yamada7, Jun-ichi Kashiwakura8, Masahiro Noda2, Masanori Ikeda1, Kunihisa Kozawa4, Akihide Ryo6, Shigemi Yoshihara9, Hiromitsu Ogata10, Yoshimichi Okayama11. 1. Department of Pediatrics, National Hospital Organization Fukuyama Medical Center, Hiroshima, Japan. 2. Infectious Disease Surveillance Center, National Institute of Infectious Diseases, Tokyo, Japan. 3. Department of Pediatrics, National Hospital Organization Yokohama Medical Center, Yokohama, Japan. 4. Department of Health Science, Gunma Prefectural Institute of Public Health and Environmental Sciences, Maebashi, Japan. 5. Allergy and Immunology Group, Research Institute of Medical Science, Division of Medical Education Planning and Development, Nihon University School of Medicine, Tokyo, Japan. 6. Department of Molecular Biodefence Research, Yokohama City University Graduate School of Medicine, Yokohama, Japan. 7. Yamada Gastroenterology Pediatric Clinic, Tochigi, Japan. 8. Laboratory for Allergic Disease, RCAI, RIKEN Center for Integrative Medical Sciences (IMS-RCAI), Yokohama, Japan. 9. Department of Pediatrics, Dokkyo Medical University, Mibu, Japan. 10. Center for Information Research, National Institute of Public Health, Saitama, Tokyo, Japan. 11. Allergy and Immunology Group, Research Institute of Medical Science, Division of Medical Education Planning and Development, Nihon University School of Medicine, Tokyo, Japan. Electronic address: okayama.yoshimichi@nihon-u.ac.jp.
Abstract
BACKGROUND: Respiratory virus-induced wheezing, such as that induced by respiratory syncytial virus (RSV) and human rhinovirus, is an important risk factor for recurrent wheezing and childhood asthma. However, no biomarkers for predicting recurrent wheezing have been identified. OBJECTIVE: We searched for predictors of recurrent wheezing using nasopharyngeal aspirates obtained from patients during the first wheezing episode who were hospitalized with an acute lower respiratory tract illness. METHODS: We enrolled 82 infants during the first wheezing episode (median age, 5.0 months) who were hospitalized for acute lower respiratory tract illness between August 2009 and June 2012 and followed these patients for 2.5 years. Nasopharyngeal aspirates and blood samples were obtained on the first day of hospitalization. Viral genomes were identified by using RT-PCR and sequencing. Levels of 33 cytokines, tryptase, IgE, anti-RSV IgE, and anti-RSV IgG were measured by using ELISAs or the Bio-Plex multiplex assay. Predictors of recurrent wheezing were examined by using a stepwise logistic regression model with backward elimination. RESULTS: Sixty percent of the patients experienced recurrent wheezing episodes. One or more viruses were detected in the nasopharynxes of 93% of the patients during the first wheezing episode. IFN-γ, IL-2, IL-9, MIP-1α, and MIP-1β levels were significantly higher among patients with recurrent wheezing than among those without recurrent wheezing (P < .05 or .01). The stepwise model demonstrated that the MIP-1α level (odds ratio, 7.72; 95% CI, 1.50-39.77; P = .015) was the strongest independent predictor of the occurrence of recurrent wheezing. CONCLUSION: An increased MIP-1α level in nasopharyngeal aspirates from patients with acute respiratory symptoms during the first wheezing episode caused by viral infections might predict recurrent wheezing.
BACKGROUND:Respiratory virus-induced wheezing, such as that induced by respiratory syncytial virus (RSV) and human rhinovirus, is an important risk factor for recurrent wheezing and childhood asthma. However, no biomarkers for predicting recurrent wheezing have been identified. OBJECTIVE: We searched for predictors of recurrent wheezing using nasopharyngeal aspirates obtained from patients during the first wheezing episode who were hospitalized with an acute lower respiratory tract illness. METHODS: We enrolled 82 infants during the first wheezing episode (median age, 5.0 months) who were hospitalized for acute lower respiratory tract illness between August 2009 and June 2012 and followed these patients for 2.5 years. Nasopharyngeal aspirates and blood samples were obtained on the first day of hospitalization. Viral genomes were identified by using RT-PCR and sequencing. Levels of 33 cytokines, tryptase, IgE, anti-RSV IgE, and anti-RSV IgG were measured by using ELISAs or the Bio-Plex multiplex assay. Predictors of recurrent wheezing were examined by using a stepwise logistic regression model with backward elimination. RESULTS: Sixty percent of the patients experienced recurrent wheezing episodes. One or more viruses were detected in the nasopharynxes of 93% of the patients during the first wheezing episode. IFN-γ, IL-2, IL-9, MIP-1α, and MIP-1β levels were significantly higher among patients with recurrent wheezing than among those without recurrent wheezing (P < .05 or .01). The stepwise model demonstrated that the MIP-1α level (odds ratio, 7.72; 95% CI, 1.50-39.77; P = .015) was the strongest independent predictor of the occurrence of recurrent wheezing. CONCLUSION: An increased MIP-1α level in nasopharyngeal aspirates from patients with acute respiratory symptoms during the first wheezing episode caused by viral infections might predict recurrent wheezing.
Authors: Kedir N Turi; Jyoti Shankar; Larry J Anderson; Devi Rajan; Kelsey Gaston; Tebeb Gebretsadik; Suman R Das; Cosby Stone; Emma K Larkin; Christian Rosas-Salazar; Steven M Brunwasser; Martin L Moore; R Stokes Peebles; Tina V Hartert Journal: Am J Respir Crit Care Med Date: 2018-10-15 Impact factor: 21.405
Authors: Tariq A Bhat; Suresh G Kalathil; Noel Leigh; Thivanka Muthumalage; Irfan Rahman; Maciej L Goniewicz; Yasmin M Thanavala Journal: Nicotine Tob Res Date: 2021-06-08 Impact factor: 4.244