Literature DB >> 26493380

Comparison of 10,11-Dehydrocurvularin Polyketide Synthases from Alternaria cinerariae and Aspergillus terreus Highlights Key Structural Motifs.

Rachel V K Cochrane1, Zhizeng Gao1, Gareth R Lambkin1, Wei Xu2, Jaclyn M Winter3, Sandra L Marcus1, Yi Tang2, John C Vederas4.   

Abstract

Iterative type I polyketide synthases (PKSs) from fungi are multifunctional enzymes that use their active sites repeatedly in a highly ordered sequence to assemble complex natural products. A phytotoxic macrolide with anticancer properties, 10,11-dehydrocurvularin (DHC), is produced by cooperation of a highly reducing (HR) iterative PKS and a non-reducing (NR) iterative PKS. We have identified the DHC gene cluster in Alternaria cinerariae, heterologously expressed the active HR PKS (Dhc3) and NR PKS (Dhc5) in yeast, and compared them to corresponding proteins that make DHC in Aspergillus terreus. Phylogenetic analysis and homology modeling of these enzymes identified variable surfaces and conserved motifs that are implicated in product formation.
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Entities:  

Keywords:  bioinformatics; biosynthesis; dehydrocurvularin; heterologous expression; polyketide

Mesh：

Substances：

Year:  2015        PMID： 26493380      PMCID： PMC4804156          DOI： 10.1002/cbic.201500428

Source DB:  PubMed          Journal:  Chembiochem        ISSN： 1439-4227            Impact factor:   3.164


  26 in total

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3.  First total syntheses and spectral data corrections of 11-alpha-methoxycurvularin and 11-beta-methoxycurvularin.

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4.  Betagamma-dehydrocurvularin and related compounds as nematicides of Pratylenchus penetrans from the fungus Aspergillus sp.

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Journal:  Biosci Biotechnol Biochem       Date:  2003-06       Impact factor: 2.043

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Journal:  Proteins       Date:  2009

6.  A concise synthetic approach to beta,gamma-dehydrocurvularin: synthesis of (+/-)-di-O-methyl-beta,gamma-dehydrocurvularin.

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Journal:  Biosci Biotechnol Biochem       Date:  2007-06       Impact factor: 2.043

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2.  Characterization of NRPS and PKS genes involved in the biosynthesis of SMs in Alternaria dauci including the phytotoxic polyketide aldaulactone.

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Journal:  Sci Rep       Date:  2022-05-17       Impact factor: 4.996

3.  Intrinsic and Extrinsic Programming of Product Chain Length and Release Mode in Fungal Collaborating Iterative Polyketide Synthases.

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Journal:  J Am Chem Soc       Date:  2020-09-23       Impact factor: 15.419
  3 in total

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