Seigo Ohba1, Yoshinori Sumita2, Mayumi Umebayashi2, Hitoshi Yoshimura3, Hisato Yoshida3, Shinpei Matsuda3, Hideki Kimura4, Izumi Asahina2, Kazuo Sano3. 1. Department of Regenerative Oral Surgery, Nagasaki University Graduate School of Biomedical Sciences, Japan; Division of Dentistry and Oral Surgery, Department of Sensory and Locomotor Medicine, Faculty of Medical Sciences, University of Fukui, Japan. Electronic address: sohba@nagasaki-u.ac.jp. 2. Department of Regenerative Oral Surgery, Nagasaki University Graduate School of Biomedical Sciences, Japan. 3. Division of Dentistry and Oral Surgery, Department of Sensory and Locomotor Medicine, Faculty of Medical Sciences, University of Fukui, Japan. 4. Division of Nephrology, Department of Medicine, Faculty of Medical Sciences, University of Fukui, Japan.
Abstract
OBJECTIVE: The aim of this study was to assess newly formed onlay bone on mouse calvarial bone using a new artificial bone material, a hydroxyapatite/collagen composite, with total blood or platelet-rich plasma. DESIGN: The hydroxyapatite/collagen composite material with normal saline, total blood or platelet-rich plasma was transplanted on mouse calvarial bone. The mice were sacrificed and the specimens were harvested four weeks after surgery. The newly formed bone area was measured on hematoxylin and eosin stained specimens using Image J software. RESULTS: The hydroxyapatite/collagen composite materials with total blood or platelet-rich plasma induced a significantly greater amount of newly formed bone than that with normal saline. Moreover, bone marrow was observed four weeks after surgery in the transplanted materials with total blood or platelet-rich plasma but not with normal saline. However, there were no significant differences in the amount of newly formed bone between materials used with total blood versus platelet-rich plasma. CONCLUSIONS: The hydroxyapatite/collagen composite material was valid for onlay bone augmentation and this material should be soaked in total blood or platelet-rich plasma prior to transplantation.
OBJECTIVE: The aim of this study was to assess newly formed onlay bone on mouse calvarial bone using a new artificial bone material, a hydroxyapatite/collagen composite, with total blood or platelet-rich plasma. DESIGN: The hydroxyapatite/collagen composite material with normal saline, total blood or platelet-rich plasma was transplanted on mouse calvarial bone. The mice were sacrificed and the specimens were harvested four weeks after surgery. The newly formed bone area was measured on hematoxylin and eosin stained specimens using Image J software. RESULTS: The hydroxyapatite/collagen composite materials with total blood or platelet-rich plasma induced a significantly greater amount of newly formed bone than that with normal saline. Moreover, bone marrow was observed four weeks after surgery in the transplanted materials with total blood or platelet-rich plasma but not with normal saline. However, there were no significant differences in the amount of newly formed bone between materials used with total blood versus platelet-rich plasma. CONCLUSIONS: The hydroxyapatite/collagen composite material was valid for onlay bone augmentation and this material should be soaked in total blood or platelet-rich plasma prior to transplantation.
Authors: Mohammed E Grawish; Lamyaa M Grawish; Hala M Grawish; Mahmoud M Grawish; Salwa A El-Negoly Journal: Tissue Eng Regen Med Date: 2020-07-03 Impact factor: 4.169
Authors: Ulrike Kuchler; Gabriel Mulinari Dos Santos; Patrick Heimel; Alexandra Stähli; Franz Josef Strauss; Stefan Tangl; Reinhard Gruber Journal: Clin Oral Implants Res Date: 2019-09-30 Impact factor: 5.021