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Literature DB >> 26491185

Accuracy of Conventional PCR Targeting the 16S rRNA Gene with the Ot-16sRF1 and Ot-16sRR1 Primers for Diagnosis of Scrub Typhus: a Case-Control Study.

Choon-Mee Kim¹, Min Keun Cho², Dong-Min Kim³, Na-Ra Yun², Seok Won Kim⁴, Sook Jin Jang⁵, Young-Joon Ahn⁶, Donghoon Lim⁷.

Abstract

We retrospectively evaluated the accuracy of conventional PCR targeting the 16S rRNA gene (16S C-PCR) using the Ot-16sRF1/Ot-16sRR1 primers for diagnosing scrub typhus. The diagnosis of Orientia tsutsugamushi infection by 16S C-PCR presented an increased sensitivity of 87.0% and specificity of 100% compared with those obtained with other targets and is thus a simple and clinically useful method with good diagnostic accuracy.

Entities: Disease

Mesh：

Substances：

Year: 2015 PMID： 26491185 PMCID： PMC4702754 DOI： 10.1128/JCM.01754-15

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

12 in total

1. Rapid and simple identification of Orientia tsutsugamushi from other group rickettsiae by duplex PCR assay using groEL gene.

Authors: Hyo-Soon Park; Jung-Hee Lee; Eun-Ju Jeong; Jung-Eun Kim; Sung-Jin Hong; Tae-Kyu Park; Tae-Yung Kim; Won-Jong Jang; Kyung-Hee Park; Bum-Joon Kim; Yoon-Hoh Kook; Seung-Hyun Lee
Journal: Microbiol Immunol Date: 2005 Impact factor: 1.955

2. Rapid diagnosis of scrub typhus in rural Thailand using polymerase chain reaction.

Authors: Piengchan Sonthayanon; Wirongrong Chierakul; Vanaporn Wuthiekanun; Stuart D Blacksell; Kriangsak Pimda; Yupin Suputtamongkol; Sasithon Pukrittayakamee; Nicholas J White; Nicholas P Day; Sharon J Peacock
Journal: Am J Trop Med Hyg Date: 2006-12 Impact factor: 2.345

3. Severe scrub typhus confirmed early via immunohistochemical staining.

Authors: Dong-Min Kim; Sung-Chul Lim; Kyung Jun Won; Yeon-Joo Choi; Kyung-Hee Park; Won-Jong Jang
Journal: Am J Trop Med Hyg Date: 2007-10 Impact factor: 2.345

4. Antigenic diversity of rickettsia tsutsugamushi: epidemiologic and ecologic significance.

Authors: B L Elisberg; J M Campbell; F M Bozeman
Journal: J Hyg Epidemiol Microbiol Immunol Date: 1968

5. Usefulness of nested PCR for the diagnosis of scrub typhus in clinical practice: A prospective study.

Authors: Dong-Min Kim; Na Ra Yun; Tae Young Yang; Ji Hyun Lee; Jong Tae Yang; Soo-Kyoung Shim; Eun-Na Choi; Mi-Yeoun Park; Seung-Hyun Lee
Journal: Am J Trop Med Hyg Date: 2006-09 Impact factor: 2.345

6. Multiple organ failure complicating probable scrub typhus.

Authors: C Cracco; C Delafosse; L Baril; Y Lefort; C Morelot; J P Derenne; F Bricaire; T Similowski
Journal: Clin Infect Dis Date: 2000-07 Impact factor: 9.079

7. Development of a quantitative real-time polymerase chain reaction assay specific for Orientia tsutsugamushi.

Authors: Ju Jiang; Teik-Chye Chan; Joseph J Temenak; Gregory A Dasch; Wei-Mei Ching; Allen L Richards
Journal: Am J Trop Med Hyg Date: 2004-04 Impact factor: 2.345

8. Novel real-time PCR assay for detection of Helicobacter pylori infection and simultaneous clarithromycin susceptibility testing of stool and biopsy specimens.

Authors: Claudia Schabereiter-Gurtner; Alexander M Hirschl; Brigitte Dragosics; Peter Hufnagl; Sonja Puz; Zsuzsanna Kovách; Manfred Rotter; Athanasios Makristathis
Journal: J Clin Microbiol Date: 2004-10 Impact factor: 5.948

Accuracy of Conventional PCR Targeting the 16S rRNA Gene with the Ot-16sRF1 and Ot-16sRR1 Primers for Diagnosis of Scrub Typhus: a Case-Control Study.

1. Rapid and simple identification of Orientia tsutsugamushi from other group rickettsiae by duplex PCR assay using groEL gene.

2. Rapid diagnosis of scrub typhus in rural Thailand using polymerase chain reaction.

3. Severe scrub typhus confirmed early via immunohistochemical staining.

4. Antigenic diversity of rickettsia tsutsugamushi: epidemiologic and ecologic significance.

5. Usefulness of nested PCR for the diagnosis of scrub typhus in clinical practice: A prospective study.

6. Multiple organ failure complicating probable scrub typhus.

7. Development of a quantitative real-time polymerase chain reaction assay specific for Orientia tsutsugamushi.

8. Novel real-time PCR assay for detection of Helicobacter pylori infection and simultaneous clarithromycin susceptibility testing of stool and biopsy specimens.

9. Scrub typhus in Himalayas.

10. Genotyping of Orientia tsutsugamushi from humans with scrub typhus, Laos.

1. Evaluation of the Diagnostic Accuracy of Antibody Assays for Patients with Scrub Typhus.

Review 2. Scrub typhus: risks, diagnostic issues, and management challenges.

3. Manifestation of anaplasmosis as cerebral infarction: a case report.

4. A case report of scrub typhus complicated with myocarditis and rhabdomyolysis.