X G Zhang1,2, B T Song3, F J Liu4, D Sun1, K X Wang1, H Qu1. 1. General Surgery Department, Qilu Hospital, Shandong University, No 107 Jinan West Wenhua Road, Jinan, 250012, China. 2. Emergency Department, The Central Hospital of Shengli Oil Field, Dongying, 257000, China. 3. General Surgery Department, Affiliated Hospital of Binzhou Medical University, Binzhou, 256603, Shandong, China. 4. General Surgery Department, Qilu Hospital, Shandong University, No 107 Jinan West Wenhua Road, Jinan, 250012, China. sdliufengjun@163.com.
Abstract
BACKGROUND: CCR6 expression is deregulated in some human malignancies and may be involved in the tumor progression. The aim of the present study was to determine the CCR6 expression in gastric cancer (GC) and to clarify its clinical significance. METHODS: We used western blotting to examine CCR6 protein expression in GC tissues and matched adjacent non-tumor tissues. Immunohistochemistry was performed on a large cohort of 372 postoperative GC samples. Chi-square test, Kaplan-Meier analysis and Cox regression model were used to analyze the data. RESULTS: Upregulated CCR6 protein expression was observed in the GC tissues by western blotting compared with the adjacent non-cancerous gastric tissues. High CCR6 expression was detected in 56.5 % (210/372) samples and significantly associated with the extracapsular extension of the tumor, tumor relapse and poor overall survival in GC (P < 0.001). Further analysis demonstrated that the CCR6 expression level stratified the patient outcome in stage II, stage III, T3/4, N positive and poorly differentiated/undifferentiated tumor subgroups. The Cox regression analysis showed that high expression of CCR6 was an independent prognostic factor for GC patients. CONCLUSIONS: CCR6 expression may be a novel biomarker for predicting clinical outcomes for GC patients.
BACKGROUND:CCR6 expression is deregulated in some humanmalignancies and may be involved in the tumor progression. The aim of the present study was to determine the CCR6 expression in gastric cancer (GC) and to clarify its clinical significance. METHODS: We used western blotting to examine CCR6 protein expression in GC tissues and matched adjacent non-tumor tissues. Immunohistochemistry was performed on a large cohort of 372 postoperative GC samples. Chi-square test, Kaplan-Meier analysis and Cox regression model were used to analyze the data. RESULTS: Upregulated CCR6 protein expression was observed in the GC tissues by western blotting compared with the adjacent non-cancerous gastric tissues. High CCR6 expression was detected in 56.5 % (210/372) samples and significantly associated with the extracapsular extension of the tumor, tumor relapse and poor overall survival in GC (P < 0.001). Further analysis demonstrated that the CCR6 expression level stratified the patient outcome in stage II, stage III, T3/4, N positive and poorly differentiated/undifferentiated tumor subgroups. The Cox regression analysis showed that high expression of CCR6 was an independent prognostic factor for GC patients. CONCLUSIONS:CCR6 expression may be a novel biomarker for predicting clinical outcomes for GC patients.
Authors: Sophie Kirshberg; Uzi Izhar; Gail Amir; Jonathan Demma; Fiona Vernea; Katia Beider; Zippora Shlomai; Hanna Wald; Gideon Zamir; Oz M Shapira; Amnon Peled; Ori Wald Journal: PLoS One Date: 2011-09-15 Impact factor: 3.240
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Authors: Pirus Ghadjar; Christoph Loddenkemper; Sarah Ellen Coupland; Andrea Stroux; Michel Noutsias; Eckhard Thiel; Frank Christoph; Kurt Miller; Carmen Scheibenbogen; Ulrich Keilholz Journal: J Cancer Res Clin Oncol Date: 2008-05-09 Impact factor: 4.553