OBJECTIVES: A fatty liver is known to have impairment of microcirculation, which is worsened after ischemia reperfusion injury (IRI). This makes most fatty grafts unsuitable for transplantation, and in the absence of real time assessment of microcirculation this selection has been at best, random. The aim of this study was to demonstrate the utility of a contrast enhanced ultrasound model in quantitative assessment of the microcirculation of a fatty liver. METHODS: We subjected fatty mice to IRI, and blood flow dynamics were assessed before and after the injury. RESULTS: There was a significant increase in the resistive and pulsatility index of the extrahepatic artery and a significant decrease in velocity of the portal vein. There was also a quantifiable decrease in the intrahepatic blood volume, blood flow, time to peak flow, and perfusion index of mice with fatty liver, suggesting that a fatty liver develops hemodynamic abnormalities after IRI, leading to increased hepatocellular injury. CONCLUSIONS: Hemodynamic abnormalities in liver can be reliably quantified using a contrast, enhanced Doppler ultrasound, which is an inexpensive technique with multiple clinical applications. It can be used to assess the quality of the fatty liver donor graft before organ retrieval; for determining live donor candidacy, for making post-IRI recovery prognosis, and for assessing the effectiveness of therapeutic interventions.
OBJECTIVES: A fatty liver is known to have impairment of microcirculation, which is worsened after ischemia reperfusion injury (IRI). This makes most fatty grafts unsuitable for transplantation, and in the absence of real time assessment of microcirculation this selection has been at best, random. The aim of this study was to demonstrate the utility of a contrast enhanced ultrasound model in quantitative assessment of the microcirculation of a fatty liver. METHODS: We subjected fatty mice to IRI, and blood flow dynamics were assessed before and after the injury. RESULTS: There was a significant increase in the resistive and pulsatility index of the extrahepatic artery and a significant decrease in velocity of the portal vein. There was also a quantifiable decrease in the intrahepatic blood volume, blood flow, time to peak flow, and perfusion index of mice with fatty liver, suggesting that a fatty liver develops hemodynamic abnormalities after IRI, leading to increased hepatocellular injury. CONCLUSIONS:Hemodynamic abnormalities in liver can be reliably quantified using a contrast, enhanced Doppler ultrasound, which is an inexpensive technique with multiple clinical applications. It can be used to assess the quality of the fatty liver donor graft before organ retrieval; for determining live donor candidacy, for making post-IRI recovery prognosis, and for assessing the effectiveness of therapeutic interventions.
Authors: Michal Vlašín; Robert Lukáč; Zuzana Kauerová; Pavel Kohout; Josef Mašek; Eliška Bartheldyová; Štěpán Koudelka; Zina Korvasová; Jana Plocková; Nikola Hronová; Jaroslav Turánek Journal: Can J Vet Res Date: 2014-04 Impact factor: 1.310
Authors: Nitika A Gupta; Vasantha L Kolachala; Rong Jiang; Carlos Abramowsky; Asha Shenoi; Astrid Kosters; Haritha Pavuluri; Frank Anania; Allan D Kirk Journal: Am J Physiol Gastrointest Liver Physiol Date: 2014-09-25 Impact factor: 4.052
Authors: Nirupama Deshpande; Amelie M Lutz; Ying Ren; Kira Foygel; Lu Tian; Michel Schneider; Reetesh Pai; Pankaj J Pasricha; Jürgen K Willmann Journal: Radiology Date: 2011-11-04 Impact factor: 11.105
Authors: Marcos Pasarín; Juan G Abraldes; Aina Rodríguez-Vilarrupla; Vincenzo La Mura; Joan Carles García-Pagán; Jaime Bosch Journal: J Hepatol Date: 2011-02-26 Impact factor: 25.083
Authors: Nitika A Gupta; Vasantha L Kolachala; Rong Jiang; Carlos Abramowsky; Rene Romero; Nimita Fifadara; Frank Anania; Stuart Knechtle; Allan Kirk Journal: Am J Pathol Date: 2012-09-05 Impact factor: 4.307
Authors: Jennifer C Sullivan; Bin Wang; Erika I Boesen; Gerard D'Angelo; Jennifer S Pollock; David M Pollock Journal: Am J Physiol Renal Physiol Date: 2009-05-06
Authors: Eric J Siskind; Fauzia Vandermeer; Tamar R Siskind; David A Bruno; Samuel Sultan; Josue Alvarez-Casas; Arielle Stafford; Barton Lane; John C Lamattina; Rolf N Barth; Steven I Hanish Journal: Int J Angiol Date: 2017-03-01
Authors: Haolu Wang; Leslie J Burke; Jatin Patel; Brian Wc Tse; Kim R Bridle; Victoria C Cogger; Xinxing Li; Xin Liu; Haotian Yang; Darrell H G Crawford; Michael S Roberts; Wenchao Gao; Xiaowen Liang Journal: Theranostics Date: 2020-05-18 Impact factor: 11.556
Authors: Daphne E DeTemple; Sebastian Cammann; Julia Bahlmann; Manuela Buettner; Alexander Heisterkamp; Florian W R Vondran; Stefan K Kalies Journal: PLoS One Date: 2020-10-15 Impact factor: 3.240