There are several errors within the text of this article.In the Cloning, expression and purification of L. donovani cysteine proteases a, b and c subsection of the Materials and Methods section, the sixth sentence should read: The PCR amplified fragments were separately cloned into NdeI/BamHI or HindIII/BamHI sites of bacterial expression vector pET28a (Novagen, Madison, USA). The ninth sentence of the same section should read: For clone confirmation, approximately 1 μg plasmid DNA from an individual miniprep was double digested with the appropriate restriction enzymes (NdeI and BamHI for cpa/b or BamHI and HindIII for cpc) and the digest loaded onto a 1% agarose gel, in parallel with the molecular weight marker: 1 kb DNA ladder (Fermentas, USA).The primer sequences for cloning cpc gene given in Table S1 should be as follows:
cpc Forward
5′-CGG GAT CC ATG GCC CTC CGC GCC AAG TCT GCG CT -3′