| Literature DB >> 26483022 |
Matthew B Lohse1, Pisiwat Kongsomboonvech2, Maria Madrigal2, Aaron D Hernday3, Clarissa J Nobile4.
Abstract
Chromatin immunoprecipitation experiments are critical to investigating the interactions between DNA and a wide range of nuclear proteins within a cell or biological sample. In this chapter we outline an optimized protocol for genome-wide chromatin immunoprecipitation that has been used successfully for several distinct morphological forms of numerous yeast species, and include an optimized method for amplification of chromatin immunoprecipitated DNA samples and hybridization to a high-density oligonucleotide tiling microarray. We also provide detailed suggestions on how to analyze the complex data obtained from these experiments.Entities:
Keywords: Candida albicans; ChIP -chip; ChIP -seq; Chromatin immunoprecipitation; Yeast
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Year: 2016 PMID： 26483022 PMCID： PMC4773921 DOI： 10.1007/978-1-4939-3079-1_10
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745