| Literature DB >> 26483019 |
Agnès Baudin-Baillieu1, Isabelle Hatin1, Rachel Legendre1, Olivier Namy2.
Abstract
Ribosome profiling is an emerging approach using deep sequencing of the mRNA part protected by the ribosome to study protein synthesis at the genome scale. This approach provides new insights into gene regulation at the translational level. In this review we describe the protocol to prepare polysomes and extract ribosome protected fragments before to deep sequence them.Keywords: Recoding; Ribo-seq; Ribosome profiling; Translation regulation
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Year: 2016 PMID: 26483019 DOI: 10.1007/978-1-4939-3079-1_7
Source DB: PubMed Journal: Methods Mol Biol ISSN: 1064-3745