Amber R Samuel1, Moeun Son2, Cande V Ananth1,3, Ronald J Wapner1. 1. a Division of Maternal-Fetal Medicine , Department of Obstetrics and Gynecology, College of Physicians and Surgeons, Columbia University , New York , NY , USA . 2. b Department of Obstetrics and Gynecology , College of Physicians and Surgeons, Columbia University , New York , NY , USA , and. 3. c Department of Epidemiology , Mailman School of Public Health, Columbia University , New York , NY , USA.
Abstract
OBJECTIVE: This study aims to assess whether the fraction of cell-free fetal DNA (cffDNA) is different at 24 h or 7 days after chorionic villus sampling (CVS), compared to subjects that do not undergo CVS. METHODS: Pregnant women undergoing CVS for genetic testing and matched subjects undergoing first trimester combined screening alone were enrolled between 11(0/7) and 13(6/7) weeks gestation. The fractions of cffDNA were compared before the procedure, 24 h after and 7 days after between CVS patients and ultrasound-only patients. RESULTS: Forty-five women underwent CVS and 45 had ultrasound alone. The women undergoing CVS were, on average, older (36.8 years versus 28.5 years, p=0.001) and had a higher baseline fraction of cffDNA than women in the comparison group (11.4% versus 9.8%, p=0.033). Both groups had a decrease in the mean fraction of cffDNA after 24 h. After 7 days, the trend of the mean fraction of cffDNA continued to decline in the CVS group but began to trend toward an increase in the ultrasound only group. CONCLUSIONS: CVS does not significantly increase the fraction of cell free fetal (placental) DNA in the maternal plasma. A downward trend in cffDNA in maternal plasma is seen at 24 h and 7 days following CVS compared to baseline.
OBJECTIVE: This study aims to assess whether the fraction of cell-free fetal DNA (cffDNA) is different at 24 h or 7 days after chorionic villus sampling (CVS), compared to subjects that do not undergo CVS. METHODS: Pregnant women undergoing CVS for genetic testing and matched subjects undergoing first trimester combined screening alone were enrolled between 11(0/7) and 13(6/7) weeks gestation. The fractions of cffDNA were compared before the procedure, 24 h after and 7 days after between CVS patients and ultrasound-only patients. RESULTS: Forty-five women underwent CVS and 45 had ultrasound alone. The women undergoing CVS were, on average, older (36.8 years versus 28.5 years, p=0.001) and had a higher baseline fraction of cffDNA than women in the comparison group (11.4% versus 9.8%, p=0.033). Both groups had a decrease in the mean fraction of cffDNA after 24 h. After 7 days, the trend of the mean fraction of cffDNA continued to decline in the CVS group but began to trend toward an increase in the ultrasound only group. CONCLUSIONS: CVS does not significantly increase the fraction of cell free fetal (placental) DNA in the maternal plasma. A downward trend in cffDNA in maternal plasma is seen at 24 h and 7 days following CVS compared to baseline.
Authors: Olle Ericsson; Tarja Ahola; Fredrik Dahl; Filip Karlsson; Fredrik Persson; Olof Karlberg; Fredrik Roos; Ida Alftrén; Björn Andersson; Emelie Barkenäs; Ani Boghos; Birgit Brandner; Jenny Dahlberg; Per-Ola Forsgren; Niels Francois; Anna Gousseva; Faizan Hakamali; Åsa Janfalk-Carlsson; Henrik Johansson; Johanna Lundgren; Atefeh Mohsenchian; Linus Olausson; Simon Olofsson; Atif Qureshi; Björn Skarpås; Peter Svahn; Anna Sävneby; Eva Åström; Anna Sahlberg; Aino Fianu-Jonasson; Jérémie Gautier; Jean-Marc Costa; Bo Jacobsson; Kypros Nicolaides Journal: Prenat Diagn Date: 2019-08-19 Impact factor: 3.050