BACKGROUND: Of all human cancers, gastric carcinoma is the one of the leading causes of death. Helicobacter pylori is considered a major etiologic agent of this disease. Spontaneously occurring gastric carcinoma is a rare diagnosis in nonhuman primates. A 2011 case report documented a high incidence of gastric adenocarcinoma in a closed colony of captive sooty mangabeys (Cercebus atys). However, H. pylori infection was not detected in these animals. MATERIALS AND METHODS: In this study, using archived formalin-fixed, paraffin-embedded stomach sections of these animals alternative methodologies were used to identify H. pylori and other non-H. pylori Helicobacter species. In addition, two additional cases of sooty mangabeys with metastatic gastric carcinoma are characterized. RESULTS: Using fluorescent in situ hybridization, we identified gastric H. suis in 75% of archived and new gastric carcinoma cases. In the two newly reported cases, H. suis and a novel Helicobacter species were detected via PCR and sequence analysis of the 16S rRNA gene. H. pylori was not identified in any of the gastric carcinoma cases via FISH and/or PCR and sequence analysis of Helicobacter spp. in DNA from of available tissues. CONCLUSIONS: This report is the first to characterize Helicobacter species infection in spontaneous gastric carcinoma with metastatic potential in nonhuman primates.
BACKGROUND: Of all humancancers, gastric carcinoma is the one of the leading causes of death. Helicobacter pylori is considered a major etiologic agent of this disease. Spontaneously occurring gastric carcinoma is a rare diagnosis in nonhuman primates. A 2011 case report documented a high incidence of gastric adenocarcinoma in a closed colony of captive sooty mangabeys (Cercebus atys). However, H. pyloriinfection was not detected in these animals. MATERIALS AND METHODS: In this study, using archived formalin-fixed, paraffin-embedded stomach sections of these animals alternative methodologies were used to identify H. pylori and other non-H. pyloriHelicobacter species. In addition, two additional cases of sooty mangabeys with metastatic gastric carcinoma are characterized. RESULTS: Using fluorescent in situ hybridization, we identified gastric H. suis in 75% of archived and new gastric carcinoma cases. In the two newly reported cases, H. suis and a novel Helicobacter species were detected via PCR and sequence analysis of the 16S rRNA gene. H. pylori was not identified in any of the gastric carcinoma cases via FISH and/or PCR and sequence analysis of Helicobacter spp. in DNA from of available tissues. CONCLUSIONS: This report is the first to characterize Helicobacter species infection in spontaneous gastric carcinoma with metastatic potential in nonhuman primates.
Authors: Prachi Sharma; Joyce K Cohen; Katherine S Paul; Cynthia L Courtney; Zachary P Johnson; Daniel C Anderson Journal: Comp Med Date: 2011-12 Impact factor: 0.982
Authors: Mee Joo; Ji Eun Kwak; Sun Hee Chang; Hanseong Kim; Je G Chi; Kyung-Ah Kim; Jeon Ho Yang; June Sung Lee; Young-Soo Moon; Kyoung-Mee Kim Journal: J Korean Med Sci Date: 2007-02 Impact factor: 2.153
Authors: Miriam E Martin; Srijak Bhatnagar; Michael D George; Bruce J Paster; Don R Canfield; Jonathan A Eisen; Jay V Solnick Journal: PLoS One Date: 2013-10-08 Impact factor: 3.240
Authors: Galit H Frydman; Kelly A Metcalf Pate; Robert P Marini; Armelle M de Laforcade; Irene Bosch; Vasudevan Bakthavatchalu; Sureshkumar Muthupalani; Alton G Swennes; Claire E Lyons; Ronald G Tompkins; James G Fox Journal: Vet Clin Pathol Date: 2017-05-18 Impact factor: 1.180