Literature DB >> 2647684

A double staining method for measuring DNA content of specific cell populations utilizing computerized microscopy.

E Kerem1, D Schwartz-Arad, E Bartfeld, N Ron, I Ariel, G Zajicek.   

Abstract

A method for identifying specific cell populations with the computerized microscope is described. This method utilizes double staining techniques using immunofluorescent antibodies and the Feulgen technique. It was applied to measure the area and DNA content of the nuclei of beta-cells in patients with Persistent Neonatal Hypoglycemia with Hyperinsulinism (PNHH). A significant increase in the area and DNA content was found in nuclei of patients compared to a control group. This method may be applied to sample cells from other tissues as well.

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Year:  1989        PMID： 2647684     DOI： 10.1007/bf00501910

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  11 in total

1.  DNA and nuclear protein measurement in isolated nuclei of human endometrium.

Authors:  P S Oud; J A Reubsaet-Veldhuizen; J B Henderik; M M Pahlplatz; H G Hermkens; J Tas; J James; G P Vooijs
Journal:  Cytometry       Date:  1986-07

2.  Diploid, tetraploid and octaploid beta cells in the islets of Langerhans of the normal human pancreas.

Authors:  M G Ehrie; F J Swartz
Journal:  Diabetes       Date:  1974-07       Impact factor: 9.461

Review 3.  The endocrine pancreas of the neonate and infant.

Authors:  R Jaffe; Y Hashida; E J Yunis
Journal:  Perspect Pediatr Pathol       Date:  1982

4.  Ploidy measurements by high-resolution cytometry.

Authors:  M Bibbo; P H Bartels; H E Dytch; G L Wied
Journal:  Anal Quant Cytol Histol       Date:  1985-06       Impact factor: 0.302

5.  Pancreatic pathology in hyperinsulinemic hypoglycemia of infancy.

Authors:  R Jaffe; Y Hashida; E J Yunis
Journal:  Lab Invest       Date:  1980-03       Impact factor: 5.662

6.  A simple method of reducing the fading of immunofluorescence during microscopy.

Authors:  G D Johnson; G M Nogueira Araujo
Journal:  J Immunol Methods       Date:  1981       Impact factor: 2.303

7.  Relationship of ploidy and chromatin condensation in the rat liver, moreover a comparison of the nuclear texture in sections and touch preparations.

Authors:  W Romen; A Rüter; K Saito; H Harms; H M Aus
Journal:  Histochemistry       Date:  1980

8.  Two graph searching techniques for boundary finding in white blood cell images.

Authors:  J M Lester; H A Williams; B A Weintraub; J F Brenner
Journal:  Comput Biol Med       Date:  1978       Impact factor: 4.589

9.  Nesidioblastosis and endocrine hyperplasia of the pancreas: a secondary phenomenon.

Authors:  W B Goudswaard; H J Houthoff; J Koudstaal; R P Zwierstra
Journal:  Hum Pathol       Date:  1986-01       Impact factor: 3.466

10.  The basic structural lesion of persistent neonatal hypoglycaemia with hyperinsulinism: deficiency of pancreatic D cells or hyperactivity of B cells?

Authors:  J Rahier; K Fält; H Müntefering; K Becker; W Gepts; S Falkmer
Journal:  Diabetologia       Date:  1984-04       Impact factor: 10.122
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