Bora Yüksel1, Umut Duygu Uzunel, Tuncay Küsbeci. 1. From the Izmir Bozyaka Training and Research Hospital, Department of Ophthalmology, Saim Çıkrıkçı Cd. No:59 Bozyaka, Izmir, Turkey.
Abstract
OBJECTIVES: To evaluate the efficacy of Eusol-C as a corneal storage medium on the survival of donor endothelium. MATERIALS AND METHODS: Twenty-seven corneas not suitable for transplant were included in this study. All donor corneas were stored in Eusol-C at 4°C. Daily donor corneal endothelial cell counting was performed with an eye bank specular microscope. All corneas were discarded after the study process. RESULTS: Mean donor age was 51.3 ± 18.8 years (range, 25-94 y). The mean duration between death and corneal excision was 9.5 ± 6.7 hours (range, 3-23 h). Mean endothelial cell density was 2195 ± 383 cells/mm² at the beginning of the preservation (range, 1361-2899 cells/mm²). Donor endothelial cell density was between 1500 to 2000 cells/mm² in 9 corneas, 2000 to 2500 in 11 corneas, 2500 to 3000 in 5, and higher than 3000 in 2 corneas at baseline. Mean endothelial cell density was found 1658 cells/mm² on the eighth day of storage, with a mean endothelial cell loss rate of 24.5%. Corneas stored 9 to 24 days in Eusol-C had a rate of endothelial cell damage of 3.1% per day. CONCLUSIONS: Although our results revealed a higher endothelial cell loss than previous reports, overall performance of Eusol-C in preserving the donor endothelium may be satisfactory for clinical use.
OBJECTIVES: To evaluate the efficacy of Eusol-C as a corneal storage medium on the survival of donor endothelium. MATERIALS AND METHODS: Twenty-seven corneas not suitable for transplant were included in this study. All donor corneas were stored in Eusol-C at 4°C. Daily donor corneal endothelial cell counting was performed with an eye bank specular microscope. All corneas were discarded after the study process. RESULTS: Mean donor age was 51.3 ± 18.8 years (range, 25-94 y). The mean duration between death and corneal excision was 9.5 ± 6.7 hours (range, 3-23 h). Mean endothelial cell density was 2195 ± 383 cells/mm² at the beginning of the preservation (range, 1361-2899 cells/mm²). Donor endothelial cell density was between 1500 to 2000 cells/mm² in 9 corneas, 2000 to 2500 in 11 corneas, 2500 to 3000 in 5, and higher than 3000 in 2 corneas at baseline. Mean endothelial cell density was found 1658 cells/mm² on the eighth day of storage, with a mean endothelial cell loss rate of 24.5%. Corneas stored 9 to 24 days in Eusol-C had a rate of endothelial cell damage of 3.1% per day. CONCLUSIONS: Although our results revealed a higher endothelial cell loss than previous reports, overall performance of Eusol-C in preserving the donor endothelium may be satisfactory for clinical use.
Authors: Thatiane Lima Sampaio; Isabela Pereira Rodrigues; Micheline Borges Lucas Cresta; Anna Cláudia de Oliveira Queiroz; Thayssa Neiva da Fonseca Victer; Daniela Ferreira Salomão Pontes; Wildo Navegantes de Araújo; Sônia Nair Báo; Maria Regina Catai Chalita Journal: Cell Tissue Bank Date: 2020-11-09 Impact factor: 1.522