Literature DB >> 26475212

TransgeneOmics--A transgenic platform for protein localization based function exploration.

Susanne Hasse1, Anthony A Hyman1, Mihail Sarov1.   

Abstract

The localization of a protein is intrinsically linked to its role in the structural and functional organization of the cell. Advances in transgenic technology have streamlined the use of protein localization as a function discovery tool. Here we review the use of large genomic DNA constructs such as bacterial artificial chromosomes as a transgenic platform for systematic tag-based protein function exploration.
Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.

Keywords:  BAC transgenesis; Genome engineering; Protein localization; Protein tagging; Recombineering

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Year:  2015        PMID： 26475212     DOI： 10.1016/j.ymeth.2015.10.005

Source DB:  PubMed          Journal:  Methods        ISSN： 1046-2023            Impact factor:   3.608


  5 in total

1.  The hypoxia response pathway promotes PEP carboxykinase and gluconeogenesis in C. elegans.

Authors:  Mehul Vora; Stephanie M Pyonteck; Tatiana Popovitchenko; Tarmie L Matlack; Aparna Prashar; Nanci S Kane; John Favate; Premal Shah; Christopher Rongo
Journal:  Nat Commun       Date:  2022-10-18       Impact factor: 17.694

2.  Construction of Gpm6a/ReelinGFPCreERT2 by BAC recombination using a specific gene in hepatic mesothelial or stellate cells.

Authors:  Hong-Bo Shi; Jin-Li Lou; Hong-Lin Shi; Feng Ren; Yu Chen; Zhong-Ping Duan
Journal:  World J Gastroenterol       Date:  2017-01-14       Impact factor: 5.742

3.  ESI mutagenesis: a one-step method for introducing mutations into bacterial artificial chromosomes.

Authors:  Arnaud Rondelet; Andrei Pozniakovsky; Devika Namboodiri; Richard Cardoso da Silva; Divya Singh; Marit Leuschner; Ina Poser; Andrea Ssykor; Julian Berlitz; Nadine Schmidt; Lea Röhder; Gerben Vader; Anthony A Hyman; Alexander W Bird
Journal:  Life Sci Alliance       Date:  2020-12-08

4.  C. elegans PEZO-1 is a mechanosensitive ion channel involved in food sensation.

Authors:  Jonathan R M Millet; Luis O Romero; Jungsoo Lee; Briar Bell; Valeria Vásquez
Journal:  J Gen Physiol       Date:  2021-12-02       Impact factor: 4.000

5.  Cas9-assisted recombineering in C. elegans: genome editing using in vivo assembly of linear DNAs.

Authors:  Alexandre Paix; Helen Schmidt; Geraldine Seydoux
Journal:  Nucleic Acids Res       Date:  2016-06-01       Impact factor: 16.971
  5 in total

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