| Literature DB >> 2647520 |
K Ando1, Y Hirata, M Shichiri, T Emori, F Marumo.
Abstract
A highly specific and sensitive radioimmunoassay has been established for measurement of human endothelin (hET) in human plasma. After extraction of plasma with an octyl-silica column, this assay allowed for detection of immunoreactive (IR) hET as low as 0.2 fmol/ml. In 16 healthy subjects, the mean concentration of plasma IR-hET was 0.6 fmol/ml. Reverse-phase HPLC coupled with radioimmunoassay revealed two major IR-hET components, one corresponding to authentic hET(1-21) and another with more hydrophilicity than hET(1-21). These data indicate that ET is a circulating vasoconstrictor hormone in man.Entities:
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Year: 1989 PMID： 2647520 DOI： 10.1016/0014-5793(89)80213-3
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124