| Literature DB >> 26472449 |
King Sun Siu1,2, Yujuan Zhang3, Xiufen Zheng1,2, James Koropatnick1,2, Wei-Ping Min4,5,6.
Abstract
The expression of a gene can be specifically downregulated by small interfering RNA (SiRNA). Modified carbon nanotubes (CNT) can be used to protect SiRNA and facilitate its entry into cells. Regardless of that, simple and efficient functionalization of CNT is lacking. Effective SiRNA delivery can be carried out using non-covalently functionalized CNT, where non-covalent (versus covalent) functionalization is simpler and more expeditious. Non-covalently functionalized single walled carbon nanotubes (SWCNT) that include a lipopolymer are described here. Polyethylenimine (PEI) conjugated to 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000] (DSPE-PEG) was generated and the products used to disperse CNT to form DSPE-PEG-PEI/CNT (DGI/C), an agent capable of facilitating SiRNA delivery to cells in vitro and organs and cells in vivo.Entities:
Keywords: Carbon nanotube s; Gene silencing; PEG; PEI; Polyethylene glycol; Polyethylenimine; RNAi; SiRNA delivery
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Year: 2016 PMID： 26472449 DOI： 10.1007/978-1-4939-3112-5_13
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745