| Literature DB >> 26471604 |
Yaqin Xu1, Fei Cai1, Zeyuan Yu2, Ling Zhang1, Xingguo Li2, Yu Yang3, Gaijie Liu1.
Abstract
Pressurised water extraction (PWE) of polysaccharides from blackcurrant fruits was investigated using a response surface methodology (RSM). The optimal conditions for PWE were: time 51min, pressure 1.6MPa, and temperature 52°C. Under these conditions, the experimental yield of Ribes nigrum L. polysaccharides (RNLP) was 11.68±0.12%, which closely agreed with the predicted value (11.77%). After preliminary purification with D4006 macroporous resin, RNLP I was obtained and its chemical characterisation was undertaken by GC, HPLC, and IR spectroscopy. RNLP I was composed of rhamnose, arabinose, xylose, mannose, galactose, and glucose with a molar ratio of 2.89:14.82:1.02:1.00:2.53:6.39 and its molecular weight was 1.49×10(4)kDa. The antioxidant activity of RNLP I was evaluated by free radical scavenging assays and a reducing power assay in vitro. RNLP I showed strong DPPH and superoxide radical scavenging activities and reducing power.Entities:
Keywords: 1,1-Diphenyl-1-picrylhydrazyl (PubChem CID: 2735032); Antioxidant activity; Ascorbic acid (PubChem CID: 54670067); Blackcurrant; Chemical characterisation; Phenanthroline (PubChem CID: 1318); Polysaccharides; Pressurised water extraction; Pyrogallol (PubChem CID: 1057); Response surface methodology; Trifluoroacetic acid (PubChem CID: 6422); d-Galacturonic acid (PubChem CID: 84740); d-Glucose (PubChem CID: 5793)
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Year: 2015 PMID： 26471604 DOI： 10.1016/j.foodchem.2015.08.061
Source DB: PubMed Journal: Food Chem ISSN： 0308-8146 Impact factor: 7.514