Literature DB >> 26468097

A General Method for Measuring Persister Levels in Escherichia coli Cultures.

Niilo Kaldalu1, Arvi Jõers1, Henri Ingelman1, Tanel Tenson2.   

Abstract

Genetically homogeneous bacterial cultures contain persisters, cells that are not killed by bactericidal antibiotics. These cells are suggested to be involved in the establishment of chronic infections. Persister levels depend on growth conditions. Here, we discuss the parameters that have to be considered when measuring persister levels and provide a sample protocol to do it.

Entities:  

Keywords:  Ampicillin; Antibiotic; Antibiotic tolerance; Fluoroquinolones; Persister

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Substances：

Year:  2016        PMID： 26468097     DOI： 10.1007/978-1-4939-2854-5_3

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  4 in total

Review 1.  In Vitro Studies of Persister Cells.

Authors:  Niilo Kaldalu; Vasili Hauryliuk; Kathryn Jane Turnbull; Agnese La Mensa; Marta Putrinš; Tanel Tenson
Journal:  Microbiol Mol Biol Rev       Date:  2020-11-11       Impact factor: 11.056

2.  Composition of the outgrowth medium modulates wake-up kinetics and ampicillin sensitivity of stringent and relaxed Escherichia coli.

Authors:  Vallo Varik; Sofia Raquel Alves Oliveira; Vasili Hauryliuk; Tanel Tenson
Journal:  Sci Rep       Date:  2016-02-29       Impact factor: 4.379

3.  Subinhibitory Concentrations of Bacteriostatic Antibiotics Induce relA-Dependent and relA-Independent Tolerance to β-Lactams.

Authors:  Pavel Kudrin; Vallo Varik; Sofia Raquel Alves Oliveira; Jelena Beljantseva; Teresa Del Peso Santos; Ievgen Dzhygyr; Dominik Rejman; Felipe Cava; Tanel Tenson; Vasili Hauryliuk
Journal:  Antimicrob Agents Chemother       Date:  2017-03-24       Impact factor: 5.191

4.  Single-cell Raman spectroscopy identifies Escherichia coli persisters and reveals their enhanced metabolic activities.

Authors:  Chuan Wang; Rongze Chen; Jian Xu; Lijian Jin
Journal:  Front Microbiol       Date:  2022-08-04       Impact factor: 6.064
  4 in total

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