Krunal Narendrakumar1, Mukta Kulkarni2, Owen Addison1, Anca Mazare3, Ita Junkar4, Patrik Schmuki3, Rachel Sammons5, Aleš Iglič2. 1. School of Dentistry, University of Birmingham, St Chad's Queensway, Birmingham B4 6NN, UK. 2. Laboratory of Biophysics, Faculty of Electrical Engineering, University of Ljubljana, Ljubljana SI-1000, Slovenia. 3. Department of Materials Science and Engineering, Chair of Surface Science and Corrosion, University of Erlangen-Nuremberg, WW4-LKO, Erlangen, Germany. 4. Jožef Stefan Institute, Jamova cesta 39, Ljubljana SI-1000, Slovenia. 5. School of Dentistry, University of Birmingham, St Chad's Queensway, Birmingham B4 6NN, UK. Electronic address: r.l.sammons@bham.ac.uk.
Abstract
OBJECTIVES: Peri-implantitis and peri-mucositis pose a severe threat to the success of dental implants. Current research focuses on the development of surfaces that inhibit biofilm formation while not inferring with tissue integration. This study compared the adherence of two oral bacterial species, Streptococcus sanguinis and Streptococcus mutans to nanostructured titanium surfaces. METHODS: The samples included TiO2 nanotubes formed by anodization of titanium foil of 100, 50 and 15nm diameter (NT15, NT50, NT100), a nanoporous (15nm pore diameter) surface and compact TiO2 control. Adherent surviving bacteria were enumerated after 1h in an artificial saliva medium containing bovine mucin. RESULTS: Lowest numbers of adherent bacteria of both species were recovered from the original titanium foil and nanoporous surface and highest numbers from the Ti100 nanotubes. Numbers of attached S. sanguinis increased in the order (NT15<NT50<NT100), correlated with increasing percentage of surface fluoride. The lowest adhesion of S. sanguinis and S. mutans on TiO2 nanostructured surfaces was observed for small diameter nanoporous surfaces which coincides with the highest osteoblast adhesion on small diameter nanotubular/nanoporous surfaces shown in previous work. SIGNIFICANCE: This study indicates that the adherence of oral streptococci can be modified by titanium anodization and nanotube diameter.
OBJECTIVES: Peri-implantitis and peri-mucositis pose a severe threat to the success of dental implants. Current research focuses on the development of surfaces that inhibit biofilm formation while not inferring with tissue integration. This study compared the adherence of two oral bacterial species, Streptococcus sanguinis and Streptococcus mutans to nanostructured titanium surfaces. METHODS: The samples included TiO2 nanotubes formed by anodization of titanium foil of 100, 50 and 15nm diameter (NT15, NT50, NT100), a nanoporous (15nm pore diameter) surface and compact TiO2 control. Adherent surviving bacteria were enumerated after 1h in an artificial saliva medium containing bovine mucin. RESULTS: Lowest numbers of adherent bacteria of both species were recovered from the original titanium foil and nanoporous surface and highest numbers from the Ti100 nanotubes. Numbers of attached S. sanguinis increased in the order (NT15<NT50<NT100), correlated with increasing percentage of surface fluoride. The lowest adhesion of S. sanguinis and S. mutans on TiO2 nanostructured surfaces was observed for small diameter nanoporous surfaces which coincides with the highest osteoblast adhesion on small diameter nanotubular/nanoporous surfaces shown in previous work. SIGNIFICANCE: This study indicates that the adherence of oral streptococci can be modified by titanium anodization and nanotube diameter.
Authors: Denver P Linklater; Vladimir A Baulin; Saulius Juodkazis; Russell J Crawford; Paul Stoodley; Elena P Ivanova Journal: Nat Rev Microbiol Date: 2020-08-17 Impact factor: 60.633
Authors: John-Jairo Aguilera-Correa; Antonio L Doadrio; Ana Conde; Maria-Angeles Arenas; Juan-Jose de-Damborenea; María Vallet-Regí; Jaime Esteban Journal: J Mater Sci Mater Med Date: 2018-07-20 Impact factor: 3.896