Wei Huang1, Ying Huang1, Meng-yao Li1, Feng Wang1, Zhi-sheng Xu1, Ai-sheng Xiong2. 1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China. 2. State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China. xiongaisheng@njau.edu.cn.
Abstract
OBJECTIVES: The DNA-binding one zinc finger (Dof) family transcription factors (TF) are involved in stress response. Dof TFs in carrot were identified and the responses of DcDof genes to abiotic stresses were analyzed. RESULTS: 46 DcDofs in carrot were identified from carrot genome database. Based on the conserved domain in Dof TF family of Arabidopsis thaliana, the DcDof TFs were divided into four classes, named class A, B, C and D. Carrot and Arabidopsis shared most motifs in the same subgroup. Real-time quantification PCR analysis showed tissue-specific expression patterns in DcDofs. DcDofs from eight subgroups responded to four abiotic stress treatments. CONCLUSIONS: The expression profiles were different with the abiotic stresses changed, indicating complicated regulatory mechanisms in Dof TF family in higher plant, and the response mechanisms of Dof genes may be influenced by different plant species.
OBJECTIVES: The DNA-binding one zinc finger (Dof) family transcription factors (TF) are involved in stress response. Dof TFs in carrot were identified and the responses of DcDof genes to abiotic stresses were analyzed. RESULTS: 46 DcDofs in carrot were identified from carrot genome database. Based on the conserved domain in Dof TF family of Arabidopsis thaliana, the DcDof TFs were divided into four classes, named class A, B, C and D. Carrot and Arabidopsis shared most motifs in the same subgroup. Real-time quantification PCR analysis showed tissue-specific expression patterns in DcDofs. DcDofs from eight subgroups responded to four abiotic stress treatments. CONCLUSIONS: The expression profiles were different with the abiotic stresses changed, indicating complicated regulatory mechanisms in Dof TF family in higher plant, and the response mechanisms of Dof genes may be influenced by different plant species.
Authors: Julio A Massange-Sánchez; Paola A Palmeros-Suárez; Eduardo Espitia-Rangel; Isaac Rodríguez-Arévalo; Lino Sánchez-Segura; Norma A Martínez-Gallardo; Fulgencio Alatorre-Cobos; Axel Tiessen; John P Délano-Frier Journal: PLoS One Date: 2016-10-17 Impact factor: 3.240