AIM: To profile the long noncoding RNA (lncRNA) expression patterns in normal lymph node (NLN) of colorectal cancer compared with paired metastatic lymph node (MLN). MATERIALS AND METHODS: MLN, NLN lncRNA and messenger RNA (mRNA) expression levels were determined by microarray in three patients; quantitative reverse transcription-polymerase chain reaction validation of 3 differentially expressed lncRNAs was conducted in another 26 patients. The lncRNAs' functions were predicted through coexpressed mRNA annotations. RESULT(S): A total of 1133 lncRNA transcripts transcripts were dysregulated in metastatic lymph node, compared with normal lymph node. The expressions of the 3 chosen lncRNAs were validated by quantitative reverse transcription-polymerase chain reaction. Functional analysis suggests that several groups of lncRNAs may participate in biological pathways related to colorectal cancer by cis- and/or trans-regulation of protein-coding genes. CONCLUSION(S): This study constitutes the first report of lncRNA expression patterns in human MLN, NLN of colorectal cancer. More than 1000 dysregulated lncRNA transcripts are found by microarray.
AIM: To profile the long noncoding RNA (lncRNA) expression patterns in normal lymph node (NLN) of colorectal cancer compared with paired metastatic lymph node (MLN). MATERIALS AND METHODS: MLN, NLN lncRNA and messenger RNA (mRNA) expression levels were determined by microarray in three patients; quantitative reverse transcription-polymerase chain reaction validation of 3 differentially expressed lncRNAs was conducted in another 26 patients. The lncRNAs' functions were predicted through coexpressed mRNA annotations. RESULT(S): A total of 1133 lncRNA transcripts transcripts were dysregulated in metastatic lymph node, compared with normal lymph node. The expressions of the 3 chosen lncRNAs were validated by quantitative reverse transcription-polymerase chain reaction. Functional analysis suggests that several groups of lncRNAs may participate in biological pathways related to colorectal cancer by cis- and/or trans-regulation of protein-coding genes. CONCLUSION(S): This study constitutes the first report of lncRNA expression patterns in human MLN, NLN of colorectal cancer. More than 1000 dysregulated lncRNA transcripts are found by microarray.