| Literature DB >> 26463389 |
Tennille Sibbritt1, Andrew Shafik1, Susan J Clark2, Thomas Preiss3,4.
Abstract
Mapping the position and quantifying the level of 5-methylcytosine (m(5)C) as a modification in different types of cellular RNA is an important objective in the emerging field of epitranscriptomics. Bisulfite conversion has long been the gold standard for detection of m(5)C in DNA but it can also be applied to RNA. Here, we detail methods for bisulfite treatment of RNA, locus-specific PCR amplification and detection of candidate sites by sequencing on the Illumina MiSeq platform.Entities:
Keywords: 5-Methylcytosine; Bisulfite conversion; Epitranscriptomics; MiSeq; Next-generation sequencing
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Year: 2016 PMID： 26463389 DOI： 10.1007/978-1-4939-3067-8_16
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745