Literature DB >> 26463377

Studying the Translatome with Polysome Profiling.

Paola Zuccotti1, Angelika Modelska2.   

Abstract

Polysome fractionation by sucrose density gradient centrifugation followed by analysis of RNA and protein is a technique that allows to understand the changes in translation of individual mRNAs as well as genome-wide effects on the translatome. Here, we describe the polysome profiling technique and RNA as well as protein isolation procedures from sucrose fractions.

Entities:  

Keywords:  Polysome; Profiling; Protein isolation; RNA isolation; Sucrose gradient; Transcriptome; Translational control; Translatome

Mesh：

Substances：

Year:  2016        PMID： 26463377     DOI： 10.1007/978-1-4939-3067-8_4

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  9 in total

1.  Measuring Translation Efficiency by RNA Immunoprecipitation of Translation Initiation Factors.

Authors:  Chris Lucchesi; Shakur Mohibi; Xinbin Chen
Journal:  Methods Mol Biol       Date:  2021

Review 2.  Exploring ribosome composition and newly synthesized proteins through proteomics and potential biomedical applications.

Authors:  Miroslava Stastna; Roberta A Gottlieb; Jennifer E Van Eyk
Journal:  Expert Rev Proteomics       Date:  2017-05-26       Impact factor: 3.940

3.  Polysome Profiling in Leishmania, Human Cells and Mouse Testis.

Authors:  Zemfira N Karamysheva; Elena B Tikhonova; Petar N Grozdanov; James C Huffman; Kristen R Baca; Alexander Karamyshev; R Brian Denison; Clinton C MacDonald; Kai Zhang; Andrey L Karamyshev
Journal:  J Vis Exp       Date:  2018-04-08       Impact factor: 1.355

4.  Translating Ribosome Affinity Purification (TRAP) of Cell Type-specific mRNA from Mouse Brain Lysates.

Authors:  Catherine L Salussolia; Kellen D Winden; Mustafa Sahin
Journal:  Bio Protoc       Date:  2022-05-05

5.  Accessing the Human Pluripotent Stem Cell Translatome by Polysome Profiling.

Authors:  Rubens Gomes-Júnior; Patrícia Shigunov; Bruno Dallagiovanna; Isabela Tiemy Pereira
Journal:  Methods Mol Biol       Date:  2022

6.  Translational control plays an important role in the adaptive heat-shock response of Streptomyces coelicolor.

Authors:  Giselda Bucca; Radhika Pothi; Andrew Hesketh; Carla Möller-Levet; David A Hodgson; Emma E Laing; Graham R Stewart; Colin P Smith
Journal:  Nucleic Acids Res       Date:  2018-06-20       Impact factor: 16.971

7.  Translation Efficiency and Degradation of ER-Associated mRNAs Modulated by ER-Anchored poly(A)-Specific Ribonuclease (PARN).

Authors:  Tian-Li Duan; Han Jiao; Guang-Jun He; Yong-Bin Yan
Journal:  Cells       Date:  2020-01-09       Impact factor: 6.600

8.  Readthrough of stop codons under limiting ABCE1 concentration involves frameshifting and inhibits nonsense-mediated mRNA decay.

Authors:  Giuditta Annibaldis; Michal Domanski; René Dreos; Lara Contu; Sarah Carl; Nina Kläy; Oliver Mühlemann
Journal:  Nucleic Acids Res       Date:  2020-10-09       Impact factor: 16.971

9.  The interactome of CLUH reveals its association to SPAG5 and its co-translational proximity to mitochondrial proteins.

Authors:  Mickaële Hémono; Alexandre Haller; Johana Chicher; Anne-Marie Duchêne; Richard Patryk Ngondo
Journal:  BMC Biol       Date:  2022-01-10       Impact factor: 7.431
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.