C Mikelson1, M J Kovach2, J Troisi3, S Symes4, D Adair5, R K Miller6, C Salafia7, K Johnson8, Z Lin8, S Richards2. 1. Department of Biological and Environmental Sciences, University of Tennessee-Chattanooga, 615 McCallie Ave., Chattanooga, TN 37403, USA. Electronic address: Colleen.mikelson@gmail.com. 2. Department of Biological and Environmental Sciences, University of Tennessee-Chattanooga, 615 McCallie Ave., Chattanooga, TN 37403, USA. 3. Department of Obstetrics and Gynecology, University of Salerno, Italy. 4. Department of Chemistry, University of Tennessee-Chattanooga, 615 McCallie Ave., Chattanooga, TN 37403, USA. 5. University of Tennessee College of Medicine, Department of Obstetrics and Gynecology, Chattanooga, TN, USA. 6. Department of Obstetrics and Gynecology, University of Rochester, School of Medicine and Dentistry, 601 Elmwood Ave, Box 668 Rochester, NY 14642, USA; Department of Environmental Medicine, University of Rochester, School of Medicine and Dentistry, 601 Elmwood Ave, Box 668 Rochester, NY 14642, USA; Department of Pathology, University of Rochester, School of Medicine and Dentistry, 601 Elmwood Ave, Box 668 Rochester, NY 14642, USA. 7. Placental Analytics LLC, Larchmont, NY, USA. 8. Environmental Sciences Program, Southern Illinois University Edwardsville, Edwardsville, IL 62026, USA.
Abstract
INTRODUCTION: Infants born below 2500 g are classified as low birth weight. Excess in utero exposure to cortisol has been linked to restricted fetal growth. Placental production of 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2) inactivates cortisol before passage into the fetus. The present study tested the hypothesis that placental 11β-HSD2 expression is positively correlated with an individualized birth weight centile and raw birth weight, and examines the relationship between metal concentrations in placental tissue and 11β-HSD2 expression. METHODS: Placentae from 191 births were collected and samples preserved to maintain mRNA profile. Placental 11β-HSD2 expression was measured via qRT-PCR. Addition samples were collected from placental tissues and uniformly dried in order to quantify 18 metals via ICP-MS (n = 160). RESULTS: A significant, positive correlation between 11β-HSD2 expression and individualized birth weight centile (p = 0.0321) and birth weight (p = 0.0243) was found. Additionally, maternal age and gestational age were positivity correlated with each other (p = 0.0321). Birth weight was significantly different with race, marital status, education and maternal tobacco use. Four metals (Co, Mn, Ni, Zn) demonstrated significant positive correlations (p < 0.05) with 11β-HSD2 expression. Sex specific differences were found; Co, Cu, Fe, Zn, and Ni were positively correlated with 11β-HSD2 expression in males only, no significant correlations were found in the female only sample. CONCLUSION: These data indicate that the growth potential of a fetus is related to the 11β-HSD2 expression in the placenta, and that 11β-HSD2 expression is related to the trace metals status of the mother.
INTRODUCTION:Infants born below 2500 g are classified as low birth weight. Excess in utero exposure to cortisol has been linked to restricted fetal growth. Placental production of 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2) inactivates cortisol before passage into the fetus. The present study tested the hypothesis that placental 11β-HSD2 expression is positively correlated with an individualized birth weight centile and raw birth weight, and examines the relationship between metal concentrations in placental tissue and 11β-HSD2 expression. METHODS: Placentae from 191 births were collected and samples preserved to maintain mRNA profile. Placental 11β-HSD2 expression was measured via qRT-PCR. Addition samples were collected from placental tissues and uniformly dried in order to quantify 18 metals via ICP-MS (n = 160). RESULTS: A significant, positive correlation between 11β-HSD2 expression and individualized birth weight centile (p = 0.0321) and birth weight (p = 0.0243) was found. Additionally, maternal age and gestational age were positivity correlated with each other (p = 0.0321). Birth weight was significantly different with race, marital status, education and maternal tobacco use. Four metals (Co, Mn, Ni, Zn) demonstrated significant positive correlations (p < 0.05) with 11β-HSD2 expression. Sex specific differences were found; Co, Cu, Fe, Zn, and Ni were positively correlated with 11β-HSD2 expression in males only, no significant correlations were found in the female only sample. CONCLUSION: These data indicate that the growth potential of a fetus is related to the 11β-HSD2 expression in the placenta, and that 11β-HSD2 expression is related to the trace metals status of the mother.
Authors: Gretchen Bandoli; Laura L Jelliffe-Pawlowski; Sky K Feuer; Liang Liang; Scott P Oltman; Randi Paynter; Kharah M Ross; Christine Dunkel Schetter; Kelli K Ryckman; Christina D Chambers Journal: J Perinatol Date: 2018-05-24 Impact factor: 2.521
Authors: Jacopo Troisi; Luigi Giugliano; Laura Sarno; Annamaria Landolfi; Sean Richards; Steven Symes; Angelo Colucci; Giuseppe Maruotti; David Adair; Marco Guida; Pasquale Martinelli; Maurizio Guida Journal: BMC Pregnancy Childbirth Date: 2019-12-05 Impact factor: 3.007