| Literature DB >> 26462854 |
Yuan Cao1, Giang K T Nguyen1, James P Tam1, Chuan-Fa Liu1.
Abstract
Using a recently discovered peptide ligase, butelase 1, we developed a novel method to access protein thioesters in good yield. We successfully combined it with native chemical ligation and sortase-mediated ligation in tandem for protein C-terminal labeling and dual-terminal labeling to exploit the orthogonality of these three ligation methods.Mesh:
Substances:
Year: 2015 PMID: 26462854 DOI: 10.1039/c5cc07227a
Source DB: PubMed Journal: Chem Commun (Camb) ISSN: 1359-7345 Impact factor: 6.222