Literature DB >> 2646152

Expression of ricin B chain in Escherichia coli.

K Hussain1, C Bowler, L M Roberts, J M Lord.   

Abstract

DNA encoding ricin B chain was fused to that encoding the E. coli OmpA signal peptide using the expression secretion vector pIN-111-ompA. When induced, E. coli cells transformed with the recombinant plasmid express ricin B chain. The recombinant product accumulates in the periplasmic space in a soluble, biologically active form.

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Year:  1989        PMID： 2646152     DOI： 10.1016/0014-5793(89)80568-x

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  6 in total

1.  Phage display of ricin B chain and its single binding domains: system for screening galactose-binding mutants.

Authors:  C Swimmer; S M Lehar; J McCafferty; D J Chiswell; W A Blättler; B C Guild
Journal:  Proc Natl Acad Sci U S A       Date:  1992-05-01       Impact factor: 11.205

2.  Ricin B chain fragments expressed in Escherichia coli are able to bind free galactose in contrast to the full length polypeptide.

Authors:  R Wales; H C Gorham; K Hussain; L M Roberts; J M Lord
Journal:  Glycoconj J       Date:  1994-08       Impact factor: 2.916

3.  [Paraoxonase 1 gene polymorphism 192Q/R in old men and long-livers from Tatars ethnic group].

Authors:  V V Pauk; I A Tuktarova; T R Nasibullin; L P Zueva; A Kh Adel'guzhina; E K Khusnutdinova; O E Mustafina
Journal:  Mol Biol (Mosk)       Date:  2007 Jul-Aug

4.  Production of Active Nonglycosylated Recombinant B-Chain of Type-2 Ribosome-Inactivating Protein from Viscum articulatum and Its Biological Effects on Peripheral Blood Mononuclear Cells.

Authors:  Tzu-Li Lu; Jing-Yuan Chuang; Jai-Sing Yang; Shau-Ting Chiu; Nai-Wan Hsiao; Mei-Chen Wu; Shih-Hsiung Wu; Ching-Hsiang Hsu
Journal:  Evid Based Complement Alternat Med       Date:  2011-03-30       Impact factor: 2.629

5.  Hsa-let-7c controls the committed differentiation of IGF-1-treated mesenchymal stem cells derived from dental pulps by targeting IGF-1R via the MAPK pathways.

Authors:  Gen-Xia Liu; Shu Ma; Yao Li; Yan Yu; Yi-Xiang Zhou; Ya-Die Lu; Lin Jin; Zi-Lu Wang; Jin-Hua Yu
Journal:  Exp Mol Med       Date:  2018-04-13       Impact factor: 8.718

6.  Sites of vulnerability on ricin B chain revealed through epitope mapping of toxin-neutralizing monoclonal antibodies.

Authors:  David J Vance; Amanda Y Poon; Nicholas J Mantis
Journal:  PLoS One       Date:  2020-11-09       Impact factor: 3.240
  6 in total

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