BACKGROUND: We identified recently esophageal cancer related gene-4 (ECRG4) as a candidate cytokine that is expressed on the surface of quiescent polymorphonuclear leukocytes (PMNs) and shed in response to ex vivo treatment with lipopolysaccharide. To investigate the potential biologic relevance of changes in cell surface ECRG4 in human samples, we performed a pilot study to examine a population of burn patients in whom blood could be analyzed prospectively. We hypothesized that cutaneous burn injury would alter cell surface expression of ECRG4 on PMNs. METHODS: Patients admitted with more than 20% total burn surface area (TBSA) (n = 10) had blood collected at the time of admission and weekly thereafter. For comparison, blood was obtained from a control group of healthy human volunteers (n = 4). We used flow cytometry to measure changes in ECRG4(+) PMNs from patients during recovery from injury. Esophageal cancer related gene-4 expression at each time point was compared with the patient's clinical status based on a Multiple Organ Dysfunction (MOD) score. RESULTS: Esophageal cancer related gene-4 was detected on the PMN surface of cells collected from healthy volunteers, however, within 48 h of admission after burn injury (n = 10 patients), the number of PMNs with cell surface ECRG4 was decreased. Esophageal cancer related gene-4 expression in PMNs was re-established over the course of patient recovery, unless complications occurred. In this case, the decrease in cell surface ECRG4(+) PMNs preceded the clinical diagnosis of infectious complications and was reflected by increased organ injury scores. CONCLUSION: From a small sample set, we were able to determine that PMN cell surface ECRG4 expression was decreased after burn injury and returned to baseline during recovery from injury. Although larger studies are needed to define the role of ECRG4 in human PMNs further, this report is the first assessment of cell surface ECRG4 protein in a patient population to support analogous findings in animal studies.
BACKGROUND: We identified recently esophageal cancer related gene-4 (ECRG4) as a candidate cytokine that is expressed on the surface of quiescent polymorphonuclear leukocytes (PMNs) and shed in response to ex vivo treatment with lipopolysaccharide. To investigate the potential biologic relevance of changes in cell surface ECRG4 in human samples, we performed a pilot study to examine a population of burn patients in whom blood could be analyzed prospectively. We hypothesized that cutaneous burn injury would alter cell surface expression of ECRG4 on PMNs. METHODS:Patients admitted with more than 20% total burn surface area (TBSA) (n = 10) had blood collected at the time of admission and weekly thereafter. For comparison, blood was obtained from a control group of healthy human volunteers (n = 4). We used flow cytometry to measure changes in ECRG4(+) PMNs from patients during recovery from injury. Esophageal cancer related gene-4 expression at each time point was compared with the patient's clinical status based on a Multiple Organ Dysfunction (MOD) score. RESULTS:Esophageal cancer related gene-4 was detected on the PMN surface of cells collected from healthy volunteers, however, within 48 h of admission after burn injury (n = 10 patients), the number of PMNs with cell surface ECRG4 was decreased. Esophageal cancer related gene-4 expression in PMNs was re-established over the course of patient recovery, unless complications occurred. In this case, the decrease in cell surface ECRG4(+) PMNs preceded the clinical diagnosis of infectious complications and was reflected by increased organ injury scores. CONCLUSION: From a small sample set, we were able to determine that PMN cell surface ECRG4 expression was decreased after burn injury and returned to baseline during recovery from injury. Although larger studies are needed to define the role of ECRG4 in human PMNs further, this report is the first assessment of cell surface ECRG4 protein in a patient population to support analogous findings in animal studies.
Authors: Hilary F Clark; Austin L Gurney; Evangeline Abaya; Kevin Baker; Daryl Baldwin; Jennifer Brush; Jian Chen; Bernard Chow; Clarissa Chui; Craig Crowley; Bridget Currell; Bethanne Deuel; Patrick Dowd; Dan Eaton; Jessica Foster; Christopher Grimaldi; Qimin Gu; Philip E Hass; Sherry Heldens; Arthur Huang; Hok Seon Kim; Laura Klimowski; Yisheng Jin; Stephanie Johnson; James Lee; Lhney Lewis; Dongzhou Liao; Melanie Mark; Edward Robbie; Celina Sanchez; Jill Schoenfeld; Somasekar Seshagiri; Laura Simmons; Jennifer Singh; Victoria Smith; Jeremy Stinson; Alicia Vagts; Richard Vandlen; Colin Watanabe; David Wieand; Kathryn Woods; Ming-Hong Xie; Daniel Yansura; Sothy Yi; Guoying Yu; Jean Yuan; Min Zhang; Zemin Zhang; Audrey Goddard; William I Wood; Paul Godowski; Alane Gray Journal: Genome Res Date: 2003-09-15 Impact factor: 9.043
Authors: Baiyu Zhou; Weihong Xu; David Herndon; Ronald Tompkins; Ronald Davis; Wenzhong Xiao; Wing Hung Wong; Mehmet Toner; H Shaw Warren; David A Schoenfeld; Laurence Rahme; Grace P McDonald-Smith; Douglas Hayden; Philip Mason; Shawn Fagan; Yong-Ming Yu; J Perren Cobb; Daniel G Remick; John A Mannick; James A Lederer; Richard L Gamelli; Geoffrey M Silver; Michael A West; Michael B Shapiro; Richard Smith; David G Camp; Weijun Qian; John Storey; Michael Mindrinos; Rob Tibshirani; Stephen Lowry; Steven Calvano; Irshad Chaudry; Michael A West; Mitchell Cohen; Ernest E Moore; Jeffrey Johnson; Lyle L Moldawer; Henry V Baker; Philip A Efron; Ulysses G J Balis; Timothy R Billiar; Juan B Ochoa; Jason L Sperry; Carol L Miller-Graziano; Asit K De; Paul E Bankey; Celeste C Finnerty; Marc G Jeschke; Joseph P Minei; Brett D Arnoldo; John L Hunt; Jureta Horton; J Perren Cobb; Bernard Brownstein; Bradley Freeman; Ronald V Maier; Avery B Nathens; Joseph Cuschieri; Nicole Gibran; Matthew Klein; Grant O'Keefe Journal: Proc Natl Acad Sci U S A Date: 2010-05-17 Impact factor: 11.205
Authors: Joseph P Minei; Joseph Cuschieri; Jason Sperry; Ernest E Moore; Michael A West; Brian G Harbrecht; Grant E O'Keefe; Mitchell J Cohen; Lyle L Moldawer; Ronald G Tompkins; Ronald V Maier Journal: Crit Care Med Date: 2012-04 Impact factor: 7.598
Authors: Steven Kao; Ashkaun Shaterian; David M Cauvi; Xitong Dang; Hyun Bae Chun; Antonio De Maio; Todd W Costantini; Raul Coimbra; Brian P Eliceiri; Andrew Baird Journal: Exp Lung Res Date: 2014-12-16 Impact factor: 2.459
Authors: Robert A Dorschner; Jisook Lee; Olga Cohen; Todd Costantini; Andrew Baird; Brian P Eliceiri Journal: Sci Adv Date: 2020-03-11 Impact factor: 14.957