| Literature DB >> 26458837 |
Hanns-Joachim Rziha1,2, Jörg Rohde3, Ralf Amann3,4.
Abstract
Orf virus (ORFV) is an epitheliotropic poxvirus, which belongs to the genus Parapoxvirus. Among them the highly attenuated, apathogenic strain D1701-V is regarded as a promising candidate for novel virus vector vaccines. Our recent work demonstrated that those ORFV-based recombinants were able to induce protective, long-lasting immunity in various hosts that are non-permissive for ORFV. In this chapter we describe procedures for the generation, selection, propagation, and titration of ORFV recombinants as well as transgene detection by PCR or immunohistochemical staining.Entities:
Keywords: Orf virus (ORFV); Parapoxvirus; Recombinant vector vaccine
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Year: 2016 PMID： 26458837 DOI： 10.1007/978-1-4939-3008-1_12
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745