Literature DB >> 26458082

A rapid, sensitive colorimetric assay for the high-throughput screening of transaminases in liquid or solid-phase.

D Baud1, N Ladkau1, T S Moody2, J M Ward3, H C Hailes1.   

Abstract

A new colorimetric method has been developed to screen transaminases using an inexpensive amine donor. The assay is sensitive, has a low level of background coloration, and can be used to identify and profile transaminase activities against aldehyde and ketone substrates in a high-throughput format. Significantly it is also amendable to solid phase colony screening.

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Year:  2015        PMID： 26458082     DOI： 10.1039/c5cc06817g

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  8 in total

1.  Direct Conversion of Hydrazones to Amines using Transaminases.

Authors:  Eve M Carter; Fabiana Subrizi; John M Ward; Tom D Sheppard; Helen C Hailes
Journal:  ChemCatChem       Date:  2021-09-17       Impact factor: 5.497

2.  Metagenomic discovery of a novel transaminase for valorization of monoaromatic compounds.

Authors:  Sandip V Pawar; Steven J Hallam; Vikramaditya G Yadav
Journal:  RSC Adv       Date:  2018-06-20       Impact factor: 4.036

3.  Detection of bacterial sulfatase activity through liquid- and solid-phase colony-based assays.

Authors:  Hey Young Yoon; Hyung Jun Kim; Soojin Jang; Jong-In Hong
Journal:  AMB Express       Date:  2017-07-11       Impact factor: 3.298

Review 4.  Transaminases for industrial biocatalysis: novel enzyme discovery.

Authors:  Stephen A Kelly; Stefan Mix; Thomas S Moody; Brendan F Gilmore
Journal:  Appl Microbiol Biotechnol       Date:  2020-04-16       Impact factor: 4.813

5.  Aminopolyols from Carbohydrates: Amination of Sugars and Sugar-Derived Tetrahydrofurans with Transaminases.

Authors:  Fabiana Subrizi; Laure Benhamou; John M Ward; Tom D Sheppard; Helen C Hailes
Journal:  Angew Chem Int Ed Engl       Date:  2019-02-14       Impact factor: 15.336

6.  Bioprospecting Reveals Class III ω-Transaminases Converting Bulky Ketones and Environmentally Relevant Polyamines.

Authors:  Cristina Coscolín; Nadine Katzke; Antonio García-Moyano; José Navarro-Fernández; David Almendral; Mónica Martínez-Martínez; Alexander Bollinger; Rafael Bargiela; Christoph Gertler; Tatyana N Chernikova; David Rojo; Coral Barbas; Hai Tran; Olga V Golyshina; Rainhard Koch; Michail M Yakimov; Gro E K Bjerga; Peter N Golyshin; Karl-Erich Jaeger; Manuel Ferrer
Journal:  Appl Environ Microbiol       Date:  2019-01-09       Impact factor: 4.792

7.  Novel transaminases from thermophiles: from discovery to application.

Authors:  Max Cárdenas-Fernández; Oliver Sinclair; John M Ward
Journal:  Microb Biotechnol       Date:  2021-10-29       Impact factor: 5.813

8.  Selective Colorimetric "Turn-On" Probe for Efficient Engineering of Iminium Biocatalysis.

Authors:  Lieuwe Biewenga; Michele Crotti; Mohammad Saifuddin; Gerrit J Poelarends
Journal:  ACS Omega       Date:  2020-01-28
  8 in total

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