AIMS: We evaluated the acute effects of different intensities of resistance exercise over endothelium-dependent vasodilatation, eNOSser1177 phosphorylation level and endothelial production of NO in superior mesenteric artery of healthy rats. MAIN METHODS: Groups: control (Ct), resistance exercise in the intensities of 30% (Ex30%), 50% (Ex50%) and 70% (Ex70%) of the maximal load established by the maximal repetition test (1RM). Exercise protocol: 15 sets of 10 repetitions. The rings of mesenteric artery were mounted in an isometric system or were prepared for further implementation of Western blot and DAF-FM techniques. KEY FINDINGS: The maximal response of the relaxation induced by insulin was not altered in the animals of the Ex30% group when compared to the Ct group. However, the animals of the Ex50% and Ex70% groups presented an increase in this response when compared to the Ct group. The eNOSser1177 phosphorylation levels showed an increase in Ex50% and Ex70% groups when compared to the Ct (1.6-fold and 3.3-fold, respectively). In the endothelial production of NO, it was observed that the Ex30% group did not show alteration in the NO production when compared to the Ct group. On the other hand, the animals exercised in the Ex50% and Ex70% groups showed increase in the NO synthesis when compared to the animals in the Ct group. SIGNIFICANCE: Our results suggest that the magnitude of these vascular endothelium adjustments is strongly related to the increase of the resistance exercise intensity from the intensity of 50% of 1 RM.
AIMS: We evaluated the acute effects of different intensities of resistance exercise over endothelium-dependent vasodilatation, eNOSser1177 phosphorylation level and endothelial production of NO in superior mesenteric artery of healthy rats. MAIN METHODS: Groups: control (Ct), resistance exercise in the intensities of 30% (Ex30%), 50% (Ex50%) and 70% (Ex70%) of the maximal load established by the maximal repetition test (1RM). Exercise protocol: 15 sets of 10 repetitions. The rings of mesenteric artery were mounted in an isometric system or were prepared for further implementation of Western blot and DAF-FM techniques. KEY FINDINGS: The maximal response of the relaxation induced by insulin was not altered in the animals of the Ex30% group when compared to the Ct group. However, the animals of the Ex50% and Ex70% groups presented an increase in this response when compared to the Ct group. The eNOSser1177 phosphorylation levels showed an increase in Ex50% and Ex70% groups when compared to the Ct (1.6-fold and 3.3-fold, respectively). In the endothelial production of NO, it was observed that the Ex30% group did not show alteration in the NO production when compared to the Ct group. On the other hand, the animals exercised in the Ex50% and Ex70% groups showed increase in the NO synthesis when compared to the animals in the Ct group. SIGNIFICANCE: Our results suggest that the magnitude of these vascular endothelium adjustments is strongly related to the increase of the resistance exercise intensity from the intensity of 50% of 1 RM.
Authors: Thássio R R Mesquita; Gianne P Campos-Mota; Virgínia S Lemos; Jader S Cruz; Itamar C G de Jesus; Enilton A Camargo; Jorge L Pesquero; João B Pesquero; Luciano Dos Santos A Capettini; Sandra Lauton-Santos Journal: Front Physiol Date: 2017-04-28 Impact factor: 4.566
Authors: Thássio R R Mesquita; Itamar C G de Jesus; Jucilene F Dos Santos; Grace K M de Almeida; Carla M L de Vasconcelos; Silvia Guatimosim; Fabrício N Macedo; Robervan V Dos Santos; José E R de Menezes-Filho; Rodrigo Miguel-Dos-Santos; Paulo T D Matos; Sérgio Scalzo; Valter J Santana-Filho; Ricardo L C Albuquerque-Júnior; Rose N Pereira-Filho; Sandra Lauton-Santos Journal: Front Pharmacol Date: 2017-05-11 Impact factor: 5.810
Authors: Fabrício N Macedo; Thassio R R Mesquita; Vitor U Melo; Marcelo M Mota; Tharciano L T B Silva; Michael N Santana; Larissa R Oliveira; Robervan V Santos; Rodrigo Miguel Dos Santos; Sandra Lauton-Santos; Marcio R V Santos; Andre S Barreto; Valter J Santana-Filho Journal: Front Physiol Date: 2016-06-28 Impact factor: 4.566
Authors: João Eliakim Dos S Araujo; Rodrigo Miguel-Dos-Santos; Fabrício N Macedo; Patrícia S Cunha; Milene Tavares Fontes; Gilson Masahiro Murata; Sandra Lauton-Santos; Valter J Santana-Filho; Ana Mara de O Silva; Angelo Roberto Antoniolli; Rui Curi; Jullyana de S S Quintans; Rosana de S S Barreto; Marcio R V Santos; Lucindo J Quintans-Junior; André S Barreto Journal: PLoS One Date: 2020-03-18 Impact factor: 3.240