The first solid phase extraction materials for selective lysophosphatidic acid (LPA) enrichment from human plasma are described. Molecularly imprinted polymers were designed, synthesized and evaluated as cartridge fillings. They enabled a relatively rapid and simple extraction protocol for LPA without any need for multiple liquid-liquid extraction steps. The five major subspecies of lysophosphatidic acid are readily separated from all other native plasma phospholipids, including those well-known to interfere with LPA quantitation, such as phosphatidylcholine and lysophosphatidylcholine. Outstanding LPA purity is obtained via these solid phase materials in a tandem extraction setup.
The first solid phase extraction materials for selective pan class="Chemical">lysophosphatidic acid (n>n class="Gene">LPA) enrichment from human plasma are described. Molecularly imprinted polymers were designed, synthesized and evaluated as cartridge fillings. They enabled a relatively rapid and simple extraction protocol for LPA without any need for multiple liquid-liquid extraction steps. The five major subspecies of lysophosphatidic acid are readily separated from all other native plasma phospholipids, including those well-known to interfere with LPA quantitation, such as phosphatidylcholine and lysophosphatidylcholine. Outstanding LPA purity is obtained via these solid phase materials in a tandem extraction setup.
Authors: Jialu Wang; Martha Sibrian-Vazquez; Jorge O Escobedo; Mark Lowry; Lei Wang; Yu-Hsuan Chu; Richard G Moore; Robert M Strongin Journal: Analyst Date: 2013-11-21 Impact factor: 4.616
Authors: Manish Sud; Eoin Fahy; Dawn Cotter; Alex Brown; Edward A Dennis; Christopher K Glass; Alfred H Merrill; Robert C Murphy; Christian R H Raetz; David W Russell; Shankar Subramaniam Journal: Nucleic Acids Res Date: 2006-11-10 Impact factor: 16.971