Sujita Khanal1, Eric T Cole2, Joongho Joh3, Shin Je Ghim4, Alfred Bennett Jenson5, Shesh N Rai6, Patrick J Trainor7, Brian S Shumway8. 1. Graduate Research Assistant, James Graham Brown Cancer Center, PhD candidate, Department of Biochemistry and Molecular Genetics, University of Louisville, Louisville, KY, USA. 2. Resident, Advanced Education in General Dentistry, University of North Carolina, Chapel Hill, NC, USA. 3. Instructor, Department of Medicine, Member, James Graham Brown Cancer Center, University of Louisville, Louisville, KY, USA. 4. Assistant Professor, Department of Medicine, James Graham Brown Cancer Center, University of Louisville, Louisville, KY, USA. 5. Senior scientist, Professor of Vaccinology, James Graham Brown Cancer Center, University of Louisville, Louisville, KY, USA. 6. Professor, Wendell Cherry Chair in Clinical Trial Research, Director, Biostatistics Shared Facility, James Graham Brown Cancer Center, Louisville, KY, USA. 7. Research Associate, Biostatistics Shared Facility, James Graham Brown Cancer Center, Louisville, KY, USA. 8. Associate Professor, Oral and Maxillofacial Pathology, Department of Surgical and Hospital Dentistry, University of Louisville, Louisville, KY, USA. Electronic address: brian.shumway@louisville.edu.
Abstract
OBJECTIVE: Human papillomavirus (HPV) typing of oral lesions microscopically consistent with multifocal epithelial hyperplasia (MEH) was performed to identify potential novel clinical presentations. STUDY DESIGN: MEH (N = 22 lesions, 17 patients) and squamous papilloma control samples (N = 9 lesions, 9 patients) were compared by using polymerase chain reaction-based HPV genotyping. Student's t tests were used to compare continuous characteristics. RESULTS: Of the study cases, 86.4% of MEH and only 11% of controls were positive for HPV (P = .0002). In MEH lesions, 45.5% contained HPV32, 36.4% HPV6, and 4.5% HPV40. MEH lesions were mostly multifocal (50%) and occurred in HIV-negative patients (81.3%). They predominated on the labial/buccal mucosa (63.3%), and there were significant differences between groups by anatomic site (P < .0001). HPV32, but not HPV6, was detected in known HIV-positive patients. CONCLUSIONS: A novel clinical presentation of MEH associated with HPV32 in HIV-negative, middle-aged to older adults is reported here. One case with HPV40 is the first to be reported. Future detection protocols should include HPV32, as it may be currently overlooked.
OBJECTIVE:Human papillomavirus (HPV) typing of oral lesions microscopically consistent with multifocal epithelial hyperplasia (MEH) was performed to identify potential novel clinical presentations. STUDY DESIGN: MEH (N = 22 lesions, 17 patients) and squamous papilloma control samples (N = 9 lesions, 9 patients) were compared by using polymerase chain reaction-based HPV genotyping. Student's t tests were used to compare continuous characteristics. RESULTS: Of the study cases, 86.4% of MEH and only 11% of controls were positive for HPV (P = .0002). In MEH lesions, 45.5% contained HPV32, 36.4% HPV6, and 4.5% HPV40. MEH lesions were mostly multifocal (50%) and occurred in HIV-negative patients (81.3%). They predominated on the labial/buccal mucosa (63.3%), and there were significant differences between groups by anatomic site (P < .0001). HPV32, but not HPV6, was detected in known HIV-positivepatients. CONCLUSIONS: A novel clinical presentation of MEH associated with HPV32 in HIV-negative, middle-aged to older adults is reported here. One case with HPV40 is the first to be reported. Future detection protocols should include HPV32, as it may be currently overlooked.
Authors: Sujita Khanal; Brian S Shumway; Maryam Zahin; Rebecca A Redman; John D Strickley; Patrick J Trainor; Shesh N Rai; Shin-Je Ghim; Alfred Bennett Jenson; Joongho Joh Journal: Oncotarget Date: 2018-07-13