Literature DB >> 26453210

Formation and Characterization of α-Synuclein Oligomers.

Wojciech Paslawski1,2, Nikolai Lorenzen1,3, Daniel E Otzen4.   

Abstract

The aggregation of α-synuclein (αSN) into oligomeric structures has received increasing interest during the last 10-15 years. The oligomers' potential involvement in Parkinson's disease makes them a promising therapeutic target. Therefore reproducible protocols to prepare and analyze oligomers are very important to allow direct comparison of results obtained by different research groups. In this chapter we present one established method to obtain αSN oligomers from a monomeric ensemble in a relatively easy manner. Also, we briefly discuss a selection of biophysical methods which allow for a quick characterization of oligomer purity and structure.

Entities:  

Keywords:  Aggregation; Biophysics; Chromatography; Oligomer; Protein; Purification; α-synuclein

Mesh：

Substances：

Year:  2016        PMID： 26453210     DOI： 10.1007/978-1-4939-2978-8_9

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  14 in total

1.  A Triple Role for a Bilayer: Using Nanoliposomes to Cross and Protect Cellular Membranes.

Authors:  Daniel E Otzen; Dina Morshedi; Hossein Mohammad-Beigi; Farhang Aliakbari
Journal:  J Membr Biol       Date:  2021-01-11       Impact factor: 1.843

2.  How epigallocatechin gallate binds and assembles oligomeric forms of human alpha-synuclein.

Authors:  Camilla B Andersen; Yuichi Yoshimura; Janni Nielsen; Daniel E Otzen; Frans A A Mulder
Journal:  J Biol Chem       Date:  2021-05-18       Impact factor: 5.157

3.  Nanomolar oligomerization and selective co-aggregation of α-synuclein pathogenic mutants revealed by single-molecule fluorescence.

Authors:  Emma Sierecki; Nichole Giles; Quill Bowden; Mark E Polinkovsky; Janina Steinbeck; Nicholas Arrioti; Diya Rahman; Akshay Bhumkar; Philip R Nicovich; Ian Ross; Robert G Parton; Till Böcking; Yann Gambin
Journal:  Sci Rep       Date:  2016-11-28       Impact factor: 4.379

4.  Rapid and ultra-sensitive quantitation of disease-associated α-synuclein seeds in brain and cerebrospinal fluid by αSyn RT-QuIC.

Authors:  Bradley R Groveman; Christina D Orrù; Andrew G Hughson; Lynne D Raymond; Gianluigi Zanusso; Bernardino Ghetti; Katrina J Campbell; Jiri Safar; Douglas Galasko; Byron Caughey
Journal:  Acta Neuropathol Commun       Date:  2018-02-09       Impact factor: 7.801

Review 5.  Are We Ready for Detecting α-Synuclein Prone to Aggregation in Patients? The Case of "Protein-Misfolding Cyclic Amplification" and "Real-Time Quaking-Induced Conversion" as Diagnostic Tools.

Authors:  Silvia Paciotti; Giovanni Bellomo; Leonardo Gatticchi; Lucilla Parnetti
Journal:  Front Neurol       Date:  2018-06-06       Impact factor: 4.003

6.  Two conformationally distinct α-synuclein oligomers share common epitopes and the ability to impair long-term potentiation.

Authors:  Femke van Diggelen; Dean Hrle; Mihaela Apetri; Gunna Christiansen; Gerhard Rammes; Armand Tepper; Daniel Erik Otzen
Journal:  PLoS One       Date:  2019-03-22       Impact factor: 3.240

7.  Reducing the Amyloidogenicity of Functional Amyloid Protein FapC Increases Its Ability To Inhibit α-Synuclein Fibrillation.

Authors:  Line Friis Bakmann Christensen; Kirstine Friis Jensen; Janni Nielsen; Brian Stougaard Vad; Gunna Christiansen; Daniel Erik Otzen
Journal:  ACS Omega       Date:  2019-02-22

8.  The Non-Fibrillating N-Terminal of α-Synuclein Binds and Co-Fibrillates with Heparin.

Authors:  Line K Skaanning; Angelo Santoro; Thomas Skamris; Jacob Hertz Martinsen; Anna Maria D'Ursi; Saskia Bucciarelli; Bente Vestergaard; Katrine Bugge; Annette Eva Langkilde; Birthe B Kragelund
Journal:  Biomolecules       Date:  2020-08-16

9.  Different Structural Conformers of Monomeric α-Synuclein Identified after Lyophilizing and Freezing.

Authors:  Amberley D Stephens; Nadezhda Nespovitaya; Maria Zacharopoulou; Clemens F Kaminski; Jonathan J Phillips; Gabriele S Kaminski Schierle
Journal:  Anal Chem       Date:  2018-05-22       Impact factor: 6.986

10.  Potent inhibitors of toxic alpha-synuclein identified via cellular time-resolved FRET biosensors.

Authors:  Anthony R Braun; Elly E Liao; Mian Horvath; Prakriti Kalra; Karen Acosta; Malaney C Young; Noah Nathan Kochen; Chih Hung Lo; Roland Brown; Michael D Evans; William C K Pomerantz; Elizabeth Rhoades; Kelvin Luk; Razvan L Cornea; David D Thomas; Jonathan N Sachs
Journal:  NPJ Parkinsons Dis       Date:  2021-06-28
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