Literature DB >> 2645172

Purification and crystallization of ferric enterobactin receptor protein, FepA, from the outer membranes of Escherichia coli UT5600/pBB2.

M A Jalal1, D van der Helm.   

Abstract

The ferric enterobactin receptor protein, FepA, was isolated and purified from the outer membranes of a genetically transformed strain of Escherichia coli (UT5600/pBB2) using anion-exchange chromatography, chromatofocusing and gel filtration. The purified protein was found to crystallize from 25 mM sodium phosphate buffer in the presence of 0.8% beta-D-octylglucoside under a range of conditions. The protein formed mostly small rods and needle-shaped crystals in the hanging drop method.

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Year:  1989        PMID： 2645172     DOI： 10.1016/0014-5793(89)80163-2

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  11 in total

1.  The plug domain of FepA, a TonB-dependent transport protein from Escherichia coli, binds its siderophore in the absence of the transmembrane barrel domain.

Authors:  K C Usher; E Ozkan; K H Gardner; J Deisenhofer
Journal:  Proc Natl Acad Sci U S A       Date:  2001-08-28       Impact factor: 11.205

2.  Double mutagenesis of a positive charge cluster in the ligand-binding site of the ferric enterobactin receptor, FepA.

Authors:  S M Newton; J S Allen; Z Cao; Z Qi; X Jiang; C Sprencel; J D Igo; S B Foster; M A Payne; P E Klebba
Journal:  Proc Natl Acad Sci U S A       Date:  1997-04-29       Impact factor: 11.205

3.  Binding characterization of the iron transport receptor from the outer membrane of Escherichia coli (FepA): differentiation between FepA and FecA.

Authors:  X H Zhou; D van der Helm; L Venkatramani
Journal:  Biometals       Date:  1995-04       Impact factor: 2.949

Review 4.  Mechanisms of TonB-catalyzed iron transport through the enteric bacterial cell envelope.

Authors:  P E Klebba; J M Rutz; J Liu; C K Murphy
Journal:  J Bioenerg Biomembr       Date:  1993-12       Impact factor: 2.945

5.  An internal affinity-tag for purification and crystallization of the siderophore receptor FhuA, integral outer membrane protein from Escherichia coli K-12.

Authors:  A D Ferguson; J Breed; K Diederichs; W Welte; J W Coulton
Journal:  Protein Sci       Date:  1998-07       Impact factor: 6.725

6.  Permeability properties of a large gated channel within the ferric enterobactin receptor, FepA.

Authors:  J Liu; J M Rutz; J B Feix; P E Klebba
Journal:  Proc Natl Acad Sci U S A       Date:  1993-11-15       Impact factor: 11.205

7.  Purification of outer membrane iron transport receptors from Escherichia coli by fast protein liquid chromatography: FepA and FecA.

Authors:  X H Zhou; D van der Helm; J Adjimani
Journal:  Biometals       Date:  1993       Impact factor: 2.949

8.  A novel purification of ferric citrate receptor (FecA) from Escherichia coli UT5600 and further characterization of its binding activity.

Authors:  X H Zhou; D van der Helm
Journal:  Biometals       Date:  1993       Impact factor: 2.949

9.  Marine amphiphilic siderophores: marinobactin structure, uptake, and microbial partitioning.

Authors:  Jennifer S Martinez; Alison Butler
Journal:  J Inorg Biochem       Date:  2007-07-16       Impact factor: 4.155

10.  Role of catecholate siderophores in gram-negative bacterial colonization of the mouse gut.

Authors:  Hualiang Pi; Shari A Jones; Lynn E Mercer; Jessica P Meador; Joyce E Caughron; Lorne Jordan; Salete M Newton; Tyrrell Conway; Phillip E Klebba
Journal:  PLoS One       Date:  2012-11-29       Impact factor: 3.240
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