IL-1βR-dependent priming of antitumor CD4+ T cells and sustained antitumor immunity after peri-tumoral treatment with MSU and mycobacteria.

Local immune-activating therapies seek to improve the presentation of tumor antigen, thereby promoting the activation of antitumor CD8+ T cells and delaying tumor growth. Surprisingly, little is known about the ability of these therapies to stimulate antitumor CD4+ T cells. We examined tumor-specific CD4+ T cell responses after peri-tumoral administration of the TLR3 agonist polyinosinic-polycytidylic acid (poly I:C), or the danger signal monosodium urate crystals in combination with Mycobacterium smegmatis (MSU + Msmeg) in mice. Both treatments delayed tumor growth, however, only MSU + Msmeg induced proliferation of tumor-specific CD4+ T cells in the draining lymph node (dLN). In line with the proliferation data, administration of MSU + Msmeg, but not poly I:C, enhanced the infiltration of CD4+FoxP3- T cells into the tumor, increased their capacity to produce IFNγ and TNF-α, and decreased PD-1 expression on tumor-infiltrating CD8+ T cells. Induction of CD4+ T cell proliferation by treatment with MSU + Msmeg required IL-1βR signaling, as it was blocked by administration of the IL-1βR antagonist Anakinra. In addition, treatment with Anakinra or with anti-CD4 also reversed the increased survival after tumor challenge in MSU + Msmeg treated mice. Thus, peri-tumoral treatment with MSU + Msmeg results in IL-1βR-dependent priming of antitumor CD4+ T cells in the LN, with consequent superior activation of CD4+ and CD8+ T cells within the tumor, and sustained antitumor activity.

cytotoxic T lymphocyte

draining lymph node

interferon γ

interleukin 1β receptor

lipopolysaccharide

major histocompatibility complex II

Mycobacterium smegmatis

monosodium urate crystals

natural killer

phosphate buffered saline

programmed cell death 1

polyinosinic-polycytidylic acid

toll-like receptor

tumor necrosis factor α

regulatory T cells

Introduction

Most current tumor immunotherapy approaches focus on CD8+ T cells, as these cells can directly and effectively eliminate tumor cells. However, CD4+ T cells are also central to optimal antitumor immunity. Co-transfer of CD4+ and CD8+ T cells enhances CD8+ effector function and therapeutic efficacy in a lymphopenic environment. Similarly, combined vaccination with CD4+ and CD8+ T-cell epitopes augments CD8+ T cell responses and improves clinical outcomes. These effects of CD4+ T cells are mediated through a variety of mechanisms. First, CD4+ T cells provide help for the priming of CD8+ T cell responses in lymphoid organs. Activated CD4+ T cells license antigen-presenting cells, enabling CD8+ T cells to become fully functional cytotoxic T lymphocytes (CTL). Subsequently, tumor-specific CD4+ T cells facilitate accumulation of CD8+ T cells at the effector site and enhance their antitumor activity. CD4+ T cells are also required for the maintenance of CD8+ T cell numbers, cytotoxic function and cytokine production within the tumor. Finally, CD4+ T-cell help can rescue CTL from activation-induced cell death, promoting effector function and memory formation.

In addition to their prominent role in the induction and maintenance of CTL responses, CD4+ T cells can also mediate CD8+ T cell-independent antitumor activity. CD4+ T cells can directly recognize and kill tumor targets, and cytokines produced by CD4+ T cells in tumors, especially IFNγ, can promote the antitumor activity of both hematopoietic and non-hematopoietic cells. These cytokine-mediated effects can occur independently of expression of MHCII on tumor cells and therefore do not require direct tumor cell recognition by the CD4+ T cells.

Several studies have shown that microbial products, including bacteria and synthetic or natural TLR agonists, promote antitumor immune responses and exhibit antitumor activity in experimental and clinical (reviewed in ref. 14) settings. The activity of these products may be further increased by combination with endogenous danger signals. Treatments that turn the tumor into a site of immune activation are highly attractive as they could be easily applied in the clinic and, if effective, activate antitumor immune responses that are tailored to the patient and of broad specificity. Several of these treatments are known to activate antitumor CD8+ T cells without requiring CD4+ responses whereas for others the capacity to activate CD4+ T cells is unknown. Recruiting the activity of both CD8+ and CD4+ T cells to the tumor should increase the impact of these immunotherapies.

We recently compared the antitumor activity of a range of immune stimulators, and found that effective treatments delayed tumor growth by activating both CD8+ T cells and NK cells. We now report that the same treatments differed in their ability to prime antitumor CD4+ T cell responses, and that this was due to their differential ability to engage IL-1βR-dependent signaling. Increased CD4+ T cell priming in the LN was associated with increased tumor infiltration of effector CD4+ T cells and a concomitant decrease in regulatory T cells (Treg), reduced CD8+ T cell exhaustion at the tumor site, and sustained retardation of tumor growth after cessation of therapy. Conversely, blocking IL-1βR or depleting CD4+ T cells abrogated the antitumor activity of MSU + Msmeg. Thus, local immunostimulatory treatments can successfully activate tumor-specific CD4+ T cells and favorably impact on the duration of antitumor CD8+ T cell responses.

Results

Local treatment with poly I:C or MSU + Msmeg delays the growth of B16.OVA tumors

We previously compared a range of immune activating agents for the ability to delay the growth of murine tumors including the B16-F1 melanoma and the 4T1 mammary carcinoma. Treatment of established tumors with poly I:C or a combination of MSU + Msmeg delayed primary tumor growth and reduced lung metastases, whereas several other agents were ineffective. Here, we evaluated the effect of these treatments on the OVA-expressing melanoma B16.OVA. Four local treatments with either poly I:C or MSU + Msmeg significantly reduced the size of B16.OVA tumors at the end of experiment (). In contrast, treatments with LPS, LPS and MSU (both not shown), MSU or Msmeg alone () did not alter tumor growth compared to PBS controls. Thus, the antitumor activity of poly I:C and MSU + Msmeg on B16.OVA melanomas was similar to that observed in B16-F1 and 4T1 tumors.

Figure 1.

Peri-tumoral treatment with poly I:C or a combination of MSU + Msmeg delays the growth of B16-F1 and B16.OVA tumors. C57BL/6 mice bearing palpable s.c. B16-F1 or B16.OVA tumors were given four peri-tumoral injections of the indicated treatments, starting on day 7 (B16-F1) or day 9 (B16.OVA) after tumor challenge and every second day thereafter. Bar graphs show tumor sizes 2 days after the fourth treatment and include all mice that underwent treatment. Bar graphs show mean + SEM for combined data from two independent experiments, each with 5 mice per group. p values refer to the comparison to the PBS group; ***, p < 0.001; **, p < 0.01; p values larger than 0.05 are not shown.

Treatment with MSU + Msmeg, but not poly I:C, primes tumor-specific CD4+ T cells in the dLN in an IL-1βR-dependent manner

To determine the effect of poly I:C and MSU + Msmeg treatment on CD4 T cell responses, we examined the proliferation of CFSE-labeled OTII T cells adoptively transferred into B16.OVA-bearing mice. As previously reported for untreated mice, no OTII proliferation was detected in the dLN of PBS-treated or poly I:C-treated mice (). In contrast, MSU + Msmeg treatment induced significant proliferation of OTII T cells, leading to a higher percentage and number of total and divided OTII cells compared to poly I:C (). Proliferation could not be induced by treating with M. smegmatis alone (not shown), and was restricted to the tumor-dLN, suggesting that tumor antigen was necessary for proliferation.

Figure 2.

Peri-tumoral treatment with MSU + Msmeg induces the proliferation of tumor-specific CD4+ T cells in dLNs. CFSE-labeled CD45.1+ OTII T cells were adoptively transferred into C57BL/6 mice bearing day 8 B16.OVA tumors. Mice were treated at the tumor site with poly I:C, MSU + Msmeg or PBS vehicle on day 9, and OTII proliferation was assessed in the tumor draining (d) and contralateral (contra) LN on day 14. OTII T cells were identified as single live CD45.1+ CD4+ Vα2+ cells. (A) Representative histograms showing CFSE dilution profiles for OTII cells in tumor-dLN. (B) Frequency and (C) number of OTII cells in LN. (D) Frequency and (E) number of divided OTII cells in LN. Bar graphs show mean + SEM for combined data from 2 independent experiments, each with 5 mice/group.

Our previous work showed that treatment with MSU + Msmeg induced detectable amounts of IL-1β in the serum, while poly I:C did not. As IL-1β is known to enhance CD4 T cell responses, we used the IL-1βR antagonist Anakinra to establish whether IL-1β signaling was critical for the ability of MSU + Msmeg to induce proliferation of tumor-specific CD4 T cells in vivo. As shown in , treatment with Anakinra decreased OTII proliferation by more than half. This suggested that IL-1β was important for the ability of MSU + Msmeg to stimulate CD4+ T cell proliferation.

Figure 3.

The proliferation of tumor-specific CD4+ T cells in MSU + Msmeg-treated mice requires responsiveness to IL-1β. CFSE-labeled OTII T cells were adoptively transferred into C57BL/6 mice bearing day 8 B16.OVA tumors. Mice were treated at the tumor site with MSU + Msmeg or PBS vehicle on day 8 and 10. The IL-1βR antagonist Anakinra was injected i.p. daily from day 8 through to the end of experiment. OTII proliferation was assessed in draining (d) and contralateral (contra) LN on day 13 using the strategy described in the legend to . (A) Frequency and (B) number of OTII cells in LN. (C) Frequency and (D) number of divided OTII cells in LN. Bar graphs show mean + SEM for combined data from 2 independent experiments, each with 5 mice/group.

Treatment with MSU + Msmeg enhances tumor infiltration by effector CD4+ T cells and reduces Treg

To assess whether increased proliferation of CD4 T cells in the dLN of MSU + Msmeg-treated mice translated into enhanced effector CD4 T cell responses in the tumor, we compared the phenotype of tumor-infiltrating CD4 T cells in treated and untreated mice. Compared to PBS, treatment with MSU + Msmeg significantly increased the frequency of CD4 T cells in the tumor (). These CD4 T cells showed an increased capacity to produce IFNγ and TNF-α, as assessed by intracellular staining without restimulation (), and increased expression of the activation markers CD69 and CD25 in the absence of FoxP3 (). In contrast, poly I:C treatment did not significantly change any of these parameters compared to PBS controls ().

Figure 4.

Treatment with MSU + Msmeg increases CD4+ T cell infiltration into the tumor and enhances effector CD4+ T cell activation. C57BL/6 (A–D) or Foxp3GFP (E) mice bearing established B16-F1 tumors were treated with PBS as a vehicle control, MSU + Msmeg or poly I:C on days 7, 9, 11, and 13 after tumor challenge. Tumor cell suspensions were analyzed by flow cytometry one day after the last treatment. (A) Percentages of CD3+CD4+ T cells in the total live tumor cell population. (B & C) Percentages of IFNγ+ (B) and TNF-α+ (C) CD4+ T cells in the tumor, as determined by intracellular staining without restimulation. (D) Frequency of intratumoral CD4+ T cells expressing the activation maker CD69. (E) Frequency of intratumoral CD4+FoxP3(GFP)- cells expressing the activation marker CD25. Bar graphs show mean + SEM for combined data from 2–3 independent experiments, each with 3–5 mice/group.

As total CD4+ T cells encompass not only effector cells but also Treg, we analyzed the CD4 T cell infiltrate in B16-F1 melanomas from Foxp3GFP mice. These mice express a FoxP3-GFP reporter which identifies tumor-infiltrating CD4 T cells with regulatory function in vitro. In PBS-treated tumors, about 20% of the CD4 T cells were FoxP3-GFP+. After MSU + Msmeg treatment, the frequency of Treg was nearly halved, but remained unchanged after poly I:C administration (). The reduction in the frequency of Treg in MSU + Msmeg vs. PBS-treated tumors was still apparent when calculated as percentage of total live cells in the tumor, suggesting that it was not simply a consequence of the increased proportion of CD4+ effectors (). Therefore, treatment with MSU + Msmeg, but not poly I:C, altered the CD4 infiltrate in tumors, increasing CD4 effector T cells, and at the same time reducing the proportion of CD4 Treg.

Figure 5.

Treatment with MSU + Msmeg reduces the frequency of tumor-infiltrating CD4+FoxP3+ Treg. C57BL/6 or Foxp3-GFP mice bearing established B16-F1 tumors were treated as in . One day after the last treatment, tumor cell suspensions were prepared and analyzed by flow cytometry. (A) Representative gating of CD4+FoxP3-GFP+ Treg in the CD4+ T cell population. Only single live CD45+CD4+ events are shown. (B) Frequency of CD4+FoxP3-GFP+ Treg in the intratumoral CD4+ T cell population. Each symbol corresponds to one mouse. (C) Frequency of CD4+FoxP3-GFP+ Treg in the total live tumor cell population. Each symbol corresponds to one mouse. Data are pooled from two independent experiments, each with 3–5 mice/group. Means and SEM are shown for each scatter plot.

Enhanced CD4+ T cell responses after MSU + Msmeg treatment reduce exhaustion of tumor-infiltrating CD8+ T cells

To assess whether the enhanced CD4 T cell response in MSU + Msmeg-treated mice influenced the quality of tumor-infiltrating T cells, we examined the expression of the exhaustion marker PD-1 on intratumoral CD4+ and CD8 T cells. In PBS-treated controls, the proportion of intratumoral CD4 and CD8 T cells that expressed high levels of PD-1 increased over time (, PBS controls, 1 treatment vs. 4 treatments). A similar increase was observed in poly I:C-treated tumors. In contrast, in MSU + Msmeg-treated tumors, the percentage of T cells expressing PD-1 remained stable after 4 treatments and was significantly lower compared to both the PBS and poly I:C-treated groups ().

Figure 6.

Treatment with MSU + Msmeg results in decreased PD-1 expression on tumor-infiltrating CD4+ and CD8+ T cells. C57BL/6 mice bearing established B16 melanomas were treated with PBS, MSU + Msmeg or poly I:C as indicated; treatment was given either once (one treatment) or four times every other day (four treatments). Mice were sacrificed one day after the last treatment, and expression of the exhaustion marker PD-1 on intratumoral T cells was assessed by flow cytometry. (A) Representative gating of PD-1-expressing CD4+ T cells in tumors is shown at the top; the percentages of PD-1-expressing intratumoral CD4+ cells are shown at the bottom. (B) As in A, except that CD8+ T cells are shown. (C) Impact of CD4+ T cell depletion by administration of anti-CD4 antibodies 36 and 12 h before the first peri-tumoral treatment on the expression of PD-1 on intratumoral CD8+ T cells. Bar graphs show mean + SEM for combined data from two independent experiments, each with 5 mice/group.

To further assess the role of CD4+ T cells in controlling expression of PD-1 on CD8+ T cells, we examined intra-tumoral CD8+ T cells in mice depleted of CD4+ T cells by anti-CD4+ antibody treatment. Compared to CD4+-sufficient mice, mice treated with anti-CD4 one day before the start of immunotherapy had much higher frequencies of PD-1+ CD8+ T cells in their tumors (), indicating that CD4+ T cells have a large impact on PD-1 expression by CD8+ T cells. However, even after CD4+ T cell depletion, treatment with MSU + Msmeg reduced PD-1 expression on CD4+ T cells compared to PBS controls, suggesting that MSU + Msmeg can also exert additional, CD4+ T cell-independent effects that help prevent CD8+ T cell exhaustion ().

MSU + Msmeg treatment induces sustained antitumor immunity in a CD4+ T cell and IL-1βR dependent manner

Activation of CD4 T cell responses and the consequent reduction of CD8 T cell exhaustion may result in prolonged antitumor activity. To investigate this possibility, we switched from the B16 melanoma model to the more immunogenic E.G7-OVA and EL-4 thymomas, as these tumor models might enable a more sensitive detection of immune responses at the later stages of tumor growth. Repeated peri-tumoral administrations of both poly I:C and MSU + Msmeg similarly delayed tumor growth during the course of treatment (day 4–14, ). However, after cessation of treatment, poly I:C-treated tumors resumed growth at a rate comparable to that of PBS-treated controls. In contrast, MSU + Msmeg-treated tumors continued to grow at a reduced rate (), and MSU + Msmeg-treated mice survived significantly longer than poly I:C-treated mice (). Depletion of CD4+ T cells just before the commencement of treatment completely abrogated the MSU + Msmeg induced antitumor activity (). In contrast, tumor growth and survival of PBS control and poly I:C treated groups was unchanged by CD4+ depletion (). Depletion of CD4+ T cells after the fourth of 6 peri-tumoral MSU + Msmeg treatments led to faster tumor growth and worse survival compared to the non-depleted MSU + Msmeg treated group (, day 10 depleted groups). These results suggest that CD4+ T cells play a critical ongoing role in MSU + Msmeg induced antitumor immunity that extends beyond the priming phase of CD8+ T cells.

Figure 7

(see previous page). CD4+ T cells and IL-1βR responsiveness are required for sustained tumor protection after treatment with MSU + Msmeg in a thymoma tumor model. C57BL/6 mice were challenged s.c. with E.G7-OVA (A, B) or EL4 thymoma cells (C–F) and given peri-tumoral treatments as indicated by the arrows. (A) Tumor growth and (B) survival of treated E.G7-OVA bearing mice is depicted. (C) Tumor growth and (D) survival of treated EL-4 bearing mice depleted of CD4+ T cells by i.p. injection of anti-CD4 antibodies on either days 3 and 4 or days 10 and 11 as indicated. (E) Tumor growth and (F) survival of treated EL-4 bearing mice given daily i.p. applications of the IL-1βR antagonist Anakinra from days 3–14. All tumor growth data are shown as mean ± SE and analyzed by 2-way ANOVA with Bonferroni's post-test. Survival was evaluated by Log-rank test with Bonferroni's correction for multiple testing. Data are pooled from 2 independent experiments, each with 5 mice/group (A, B, E, F) or 3–5 mice/group (C, D).

We also assessed the effect of blocking IL-1βR on tumor growth and the effect of immunotherapy. In line with our observation that IL-1βR activity was necessary for the priming of tumor-specific CD4+ T cells (), blocking IL-1βR during MSU + Msmeg treatment of EL-4 thymomas abolished the antitumor effect (). In contrast, tumor growth and survival in poly I:C treated groups were not affected by IL-1βR blockade ().

These data show that the sustained antitumor activity conferred by treatment with MSU + Msmeg requires IL-1βR signaling and the ongoing presence of CD4+ T cells, thus conferring a significant host survival benefit compared to treatments, such as polyI:C, that fail to support CD4+ T cell priming.

Discussion

In this study we compare different immune activating agents for ability to support the priming of CD4+ T cell responses to tumor-derived antigens. Previous work demonstrated that the OVA-expressing tumors B16.OVA and E.G7-OVA are unable to induce the proliferation of OVA-specific OTII CD4+ T cells in vivo, suggesting a defective function of tumor-associated DC. Here we report that treatment with MSU + Msmeg, but not poly I:C, can restore CD4+ T cell priming and increase tumor homing and intratumoral cytokine production by CD4+ T cells. We also show that increased CD4+ T cell priming by MSU + Msmeg was associated with decreased proportions of intratumoral Treg, and reduced expression of PD-1 on CD4+ and CD8+ T cells in tumors. Therefore, treatment with selected immune activating agents can enhance presentation of tumor antigen, and restore immune reactivity in the tumor context.

We previously reported that MSU + Msmeg and poly I:C can both delay tumor growth by a mechanism which requires intact CD8+ and NK cell responses. However, as shown in this paper, only MSU + Msmeg supports CD4+ T cell priming and requires CD4+ T cells for its antitumor effect. In contrast, CD4+ T cell responses were not involved in the antitumor effect of poly I:C, as also described in the literature using a malignant mesothelioma tumor model. The differential capacity to induce CD4+ T cell priming can be explained by the selective ability of MSU + Msmeg to elevate the levels of IL-1β in serum, as blocking IL-1β signaling in vivo using the IL-1βR antagonist Anakinra significantly reduced CD4+ T cell proliferation and selectively abrogated the MSU + Msmeg but not the poly I:C induced antitumor effect. IL-1β is known to enhance CD4+ T cell responses and promotes induction of antitumor immunity, however, its effects on CD4+ T cell responses have not been examined in a tumor context. IL-1β can enhance CD4+ T cell priming both via effects on antigen-presenting cells and through direct signaling in CD4+ T cells, leading to increased proliferation and cytokine production. Furthermore, IL-1β can activate MyD88 signaling in CD4+ T cells, rendering them refractory to Treg-mediated suppression and increasing their effector function. Any of these IL-1β effects could be contributing to the improved CD4+ T cell response we report here.

In addition to increasing the proportion of effector CD4+ T cells in tumors, MSU + Msmeg treatment also reduced the frequency of Treg. This lower frequency may have been purely due to reduced Treg accumulation in the tumor. It is also possible that MSU + Msmeg treatment maintained T effector function and reduced conversion of effector T cells to Treg. Alternatively, MSU + Msmeg treatment may reprogram induced Treg to an effector-like phenotype with downregulation of FoxP3-GFP expression. Downregulation of FoxP3 by IL-1β and IL-6 has been reported in an elegant study using reporter mice, and could also be induced by vaccination with antigen in CFA. Therefore, the induction of IL-1β by MSU + Msmeg, possibly in combination with other pro-inflammatory signals also elicited by this treatment, may be a contributing factor in the reprogramming of Treg to CD4+ effector cells. Recent evidence suggests that in some situations reprogrammed Treg may be essential for providing help for antitumor CD8+ T cell responses and effective vaccination.

The concomitant increase in tumor-infiltrating effector T cells and decrease in Treg in MSU + Msmeg-treated mice resulted in an increased T effector/Treg ratio in tumors. It has been shown that the ratio of T effectors to Treg, rather than just activation of effector cells, is critical for improved antitumor immunity in mice. In addition, clinical studies in different types of cancer report improved survival with increased T effector to Treg ratios even when the absolute counts of Treg or T effector cells were not indicative of outcome. Thus the relative presence of effector cells over suppressive cells in tumors is critical for successful antitumor immunity, and treatments such as MSU + Msmeg, which affect both effector and Treg, offer a particularly promising approach to tumor immunotherapy.

We have previously shown that MSU + Msmeg and poly I:C could both stimulate tumor-specific CD8+ T cell proliferation and CD8+ T cell infiltration into tumors. However, poly I:C was unable to stimulate CD4+ T cell priming and its antitumor activity was unaltered by CD4+ T cell depletion. As also proposed by others, these observations suggest that poly I:C is capable of inducing some level of CD4-independent CD8+ T cell priming. The extent of CD8+ T cell priming and tumor infiltration, however, was more pronounced in response to MSU + Msmeg, and in this case depletion of CD4+ T cells before or during treatment led to a complete or partial loss of antitumor activity, respectively. These findings are consistent with a report by Marzo et al., who found that CD4+ T cells were dispensable for CD8+ T cell priming, but were required for maintenance of the CD8+ response including tumor infiltration and cytokine production.

The sustained antitumor activity of CD8+ T cells in the presence of CD4+ T cells might result, at least in part, from decreased expression of exhaustion markers such as PD-1 on CD8+ T cells. In our experiments on untreated mice, the proportion of PD-1-expressing tumor-infiltrating CD8+ T cells increased substantially with tumor progression. This increase was not changed after poly I:C treatment, but was significantly reduced in response to MSU + Msmeg. Depletion of CD4+ T cells greatly increased the percentage of PD-1-expressing intratumoral CD8+ T cells, indicating that CD4+ T cells indeed play a critical role in preventing PD-1 upregulation on CD8+ effectors. However, in CD4+-depleted mice, MSU + Msmeg-treated tumors still showed a reduced frequency of PD-1high CD8+ T cells compared to PBS controls. This suggests that MSU + Msmeg can maintain CD8+ T cell function through additional mechanisms that do not require CD4+ T cells, possibly including the effects of cytokines and/or IL-1β on DC or directly on CD8+ T cells. Consistent with an improved maintenance of antitumor CD8+ T cell activity, we observed sustained antitumor activity against murine thymomas only after MSU + Msmeg, but not poly I:C, treatment, and this was CD4+ T cell dependent. Similarly, other authors reported increased antitumor CD8+ responses in mice treated with poly I:C and anti-PD-L1 antibodies.

In parallel to its effect on CD8+ T cells, MSU + Msmeg treatment also kept the proportion of PD-1-expressing CD4+ T cells low throughout tumor progression. Although the association of PD-1 expression with exhaustion is not as firmly established in CD4+ T cells as in CD8+ T cells, several studies have now linked functional impairment of CD4+ T cells with high levels of PD-1 expression. For example, in a model of recurrent melanoma, blocking the PD-1 ligand PD-L1 in combination with Treg depletion restored CD4+ effector function and resulted in tumor eradication in the absence of CD8+ T cells. These findings illustrate the functional importance of PD-1 expression on tumor-infiltrating CD4+ T cells. Recent successes in clinical trials blocking PD-1 or its ligands further underscore the significance of this pathway for the activation of effective antitumor immunity.

In conclusion, we show that treatment with MSU + Msmeg leads to the activation of a substantial CD4+ T cell response, reduced Treg infiltration in tumors, improved CD8+ T cell activity, and reduced T cell exhaustion resulting in sustained antitumor immunity. MSU has been found to be safe as a vaccine adjuvant for use in humans. Mycobacteria such as Bacillus Calmette-Guérin are already used in the clinic for the treatment of bladder cancer, and their safety in immune-compromised cancer patients can be further improved by replacing with non-pathogen derived species such as Msmeg or by heat-killing. We conclude that, on the basis of its efficacy and safety profile, MSU + Msmeg would be a useful basis for immune stimulatory treatments, also in combination with other immunotherapies or chemotherapy, to promote and sustain anticancer immunity.

Materials and Methods

Mice

All mice were bred at the Malaghan Institute of Medical Research Biomedical Research Unit and were age and sex-matched within experiments. C57BL/6J mice were originally from Jackson Laboratories, USA; B6.SJL-Ptrprca mice were from the Animal Resource Centre, Canning Vale, Western Australia. Foxp3GFP mice and OTII mice expressing a transgenic TCR specific for I-Ab+OVA323–339 were originally provided by Drs. A Rudensky and F Carbone, respectively. All experimental protocols were approved by the Victoria University of Wellington Animal Ethics Committee.

Tumor cell lines and tumor challenge

The B16-F1 melanoma and E.G7 OVA-expressing thymoma were obtained from the American Type Culture Collection, Manassas, VA. The B16.OVA melanoma was kindly provided by Dr Roslyn Kemp, Trudeau Institute. EL-4 thymoma cells were a gift from Prof. Michael Berridge, Malaghan Institute of Medical Research. All cell lines were maintained in complete Iscove's modified Dulbecco medium as described. Extended in vitro passaging was avoided. Mice were injected with either 105 (B16-F1, B16.OVA) or 5 × 105 (E.G7-OVA, EL-4) cells s.c. into the flank. Tumor size and survival were calculated as described.

Peri-tumoral treatments

Treatments of 50 µg poly I:C (LMW, Invivogen) or 2 × 106 CFU Mycobacterium smegmatis (Msmeg mc2155) combined with 250 µg MSU (endotoxin < 0.01 EU/10 mg) were given in a total volume of 100 µl PBS injected in the area directly adjacent to the tumors as described. PBS (Invitrogen) was used as a vehicle control. Treatment was started once the tumor became palpable (day 4–9, depending on tumor and experiment) and given every second day for 4 to 6 times. This schedule was chosen on the basis of preliminary experiments comparing tumor size and survival after treatment.

Flow cytometry

Lymph nodes (LN) or tumors were digested using DNase I and Liberase TL (Roche). Single cell preparations were resuspended in Flow buffer (PBS with 10mM EDTA, 2% FBS and 0.01% NaN3) and pretreated with anti-mouse CD16/32 (2.4G2) before staining with antibodies specific for the following markers: CD45 (30F11), CD45.1 (A20), CD4 (RM4–5), CD3ϵ (2C11), CD8α (53–6.7), CD25 (PC61), CD69 (H1.2F3), Vα2 (B20.1) (all BD Biosciences), PD-1 (RMP1–30) from Biolegend and CD4 (GK1.5) prepared in-house. Streptavidin-PE or PE-Texas-Red (BD Biosciences) were used to reveal biotinylated antibodies. Dead cells were identified by DAPI or Live/Dead Fixable blue (Invitrogen) exclusion. For intracellular cytokine staining, cell suspensions were incubated for 6 h in Golgi Stop without restimulation. Cells were then stained for surface markers, followed by intracellular staining with anti-IFNγ (XMG1.2) and anti-TNF-α (MP6-XT22) antibodies, or isotype controls (R3–34 or EBRG1), using the BD Cytofix/Cytoperm kit (all from BD Biosciences). Acquisition was performed on a BD LSRII SORP (Becton Dickinson) and data were analyzed using FlowJo version 9.7.4 (Tree Star).

In vivo T cell proliferation assay

Anti-CD4 microbeads (Miltenyi Biotech) were used to positively select CD4+ T cells from spleens and LNs of OTII x B6.SJL-Ptprca mice. CD4+ T cells were subsequently labeled with CFSE as described and 1 – 2 × 106 cells were injected i.v. into C57BL/6J hosts bearing established B16.OVA tumors. OTII proliferation was assessed in tumor-dLNs 5 d later.

CD4+ T cell depletion

CD4+ T cells were depleted by i.p. injection of 2 × 250µg of purified anti-CD4 (GK1.5) antibody as indicated in the figures. Depletion of CD4+ T cells was assessed in blood during the experiment and in some tumors on day 15, and was consistently >92%.

IL-1βR blocking

For IL-1βR blockade, mice were given daily i.p. injections of 100 mg/kg of the IL-1βR antagonist Anakinra (Swedish Orphan Biovitrum AB, Stockholm, Sweden) in sterile PBS on the days indicated in the figures.

Statistics

Statistical analyses were performed using Prism 5.0 software (GraphPad). Means ± SE are shown in all graphs. Unless otherwise indicated, data were compared using the Kruskal–Wallis test with Dunn's multiple comparison test. Tumor growth data was analyzed by 2-way-ANOVA with Bonferroni's post test. Survival data was analyzed using the log-rank test with Bonferroni's correction for multiple testing. Differences of p < 0.05 were deemed significant (*), p < 0.01 very significant (**) and p < 0.001 extremely significant (***).