Literature DB >> 26450393

Evaluation of Pregnancy Malaria Vaccine Candidates: The Binding Inhibition Assay.

Tracy Saveria1, Patrick E Duffy2, Michal Fried3.   

Abstract

The parasite-binding inhibition assay is designed to evaluate the acquisition of naturally acquired functional antibodies that block Plasmodium falciparum binding to endothelial or placental receptors. The assay is also used to assess functional activity by antibodies induced by immunization, for example antibodies raised against pregnancy malaria vaccine candidates like VAR2CSA. Here we describe a plate-based assay to measure the levels of adhesion-blocking antibodies. This assay format can be adapted to any lab that is minimally equipped for short-term parasite culture.

Entities:  

Keywords:  Binding inhibition assay; CSA; IgG purification; Plate-based assay

Mesh：

Substances：

Year:  2015        PMID： 26450393      PMCID： PMC5634520          DOI： 10.1007/978-1-4939-2815-6_19

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  8 in total

Review 1.  Epidemiology and burden of malaria in pregnancy.

Authors:  Meghna Desai; Feiko O ter Kuile; François Nosten; Rose McGready; Kwame Asamoa; Bernard Brabin; Robert D Newman
Journal:  Lancet Infect Dis       Date:  2007-02       Impact factor: 25.071

2.  Induction of adhesion-inhibitory antibodies against placental Plasmodium falciparum parasites by using single domains of VAR2CSA.

Authors:  Morten A Nielsen; Vera V Pinto; Mafalda Resende; Madeleine Dahlbäck; Sisse B Ditlev; Thor G Theander; Ali Salanti
Journal:  Infect Immun       Date:  2009-03-23       Impact factor: 3.441

3.  Pregnancy malaria: cryptic disease, apparent solution.

Authors:  Patrick Emmet Duffy; Michal Fried
Journal:  Mem Inst Oswaldo Cruz       Date:  2011-08       Impact factor: 2.743

4.  Maternal antibodies block malaria.

Authors:  M Fried; F Nosten; A Brockman; B J Brabin; P E Duffy
Journal:  Nature       Date:  1998-10-29       Impact factor: 49.962

5.  Strain-transcendent immune response to recombinant Var2CSA DBL5-ε domain block P. falciparum adhesion to placenta-derived BeWo cells under flow conditions.

Authors:  Pablo Fernandez; Stéphane Petres; Salaheddine Mécheri; Jürg Gysin; Artur Scherf
Journal:  PLoS One       Date:  2010-09-03       Impact factor: 3.240

6.  Selective upregulation of a single distinctly structured var gene in chondroitin sulphate A-adhering Plasmodium falciparum involved in pregnancy-associated malaria.

Authors:  Ali Salanti; Trine Staalsoe; Thomas Lavstsen; Anja T R Jensen; M P Kordai Sowa; David E Arnot; Lars Hviid; Thor G Theander
Journal:  Mol Microbiol       Date:  2003-07       Impact factor: 3.501

7.  Malaria infection of the placenta in The Gambia, West Africa; its incidence and relationship to stillbirth, birthweight and placental weight.

Authors:  I A McGregor; M E Wilson; W Z Billewicz
Journal:  Trans R Soc Trop Med Hyg       Date:  1983       Impact factor: 2.184

8.  Adherence of Plasmodium falciparum to chondroitin sulfate A in the human placenta.

Authors:  M Fried; P E Duffy
Journal:  Science       Date:  1996-06-07       Impact factor: 47.728
  8 in total
  2 in total

1.  Optimization of in vitro trophoblast assay for real-time impedimetric sensing of trophoblast-erythrocyte interactions in Plasmodium falciparum malaria.

Authors:  Jia Liu; Olga Chesnokova; Irina Oleinikov; Yuhao Qiang; Andrew V Oleinikov; E Du
Journal:  Anal Bioanal Chem       Date:  2020-01-27       Impact factor: 4.142

2.  Clinical development of placental malaria vaccines and immunoassays harmonization: a workshop report.

Authors:  Arnaud Chêne; Sophie Houard; Morten A Nielsen; Sophia Hundt; Flavia D'Alessio; Sodiomon B Sirima; Adrian J F Luty; Patrick Duffy; Odile Leroy; Benoit Gamain; Nicola K Viebig
Journal:  Malar J       Date:  2016-09-17       Impact factor: 2.979
  2 in total

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