| Literature DB >> 26450390 |
Abstract
The quantification of single cell interferon-gamma (IFN-γ) release for assessing cellular immune responses using the Enzyme-linked immunospot (ELISPOT) assay is an invaluable technique in immunology. Peripheral blood mononuclear cells (PBMC) are stimulated in vitro with recombinant proteins, peptides and recently whole malaria organisms. Stimulation may be short term (20-36 h) or long term (cultured ELISpot, up to 7 days). ELISpot is also able to quantify other cytokines secreted by antigen-specific T-cells, such as interleukin-2, interleukin-5, and other interleukins. ELISpot is playing an important role especially in vaccine research studies.Entities:
Keywords: Cytokines; ELISPOT; IFN-γ; Interferon-gamma; PBMC; Peripheral blood mononuclear cells; T cells
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Year: 2015 PMID： 26450390 DOI： 10.1007/978-1-4939-2815-6_16
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745