Keisuke Goto1, Toshihiro Takai2, Takaya Fukumoto3, Takashi Anan3, Tetsunori Kimura3, Shin-ichi Ansai4, Yoshimi Oshitani5, Yozo Murata2, Toshiko Sakuma6, Takanori Hirose6. 1. Department of Diagnostic Pathology, Kainan Hospital, Yatomi, Japan. 2. Department of Dermatology, Hyogo Cancer Center, Akashi, Japan. 3. Sapporo Dermatopathology Institute, Sapporo, Japan. 4. Division of Dermatology, Musashi Kosugi-Hospital, Nippon Medical School, Kawasaki, Japan. 5. Department of Dermatology, Kainan Hospital, Yatomi, Japan. 6. Department of Diagnostic Pathology, Hyogo Cancer Center, Akashi, Japan.
Abstract
BACKGROUND: Distinguishing porocarcinoma from squamous cell carcinoma (SCC) is clinically significant but can pose a diagnostic dilemma. The present study sought to confirm the diagnostic utility of CD117 immunohistochemistry in distinguishing porocarcinoma from SCC and to examine histologic, carcinoembryonic antigen (CEA) immunohistochemical and CA19-9 immunohistochemical differences between these tumors. METHODS: Immunostaining with anti-CD117, anti-CEA and anti-CA19-9 antibodies was performed for 22 porocarcinomas and 31 SCCs. The extent of CD117, CEA and CA19-9 staining was classified as negative (<1%), rarely positive (1-4%), focally positive (5-29%) or diffusely positive (30-100%). CD117 staining intensity was semi-quantitatively graded as weak, moderate or strong. RESULTS: All (100%) porocarcinomas were positive for CD117, with mainly focal (8/22) or diffuse (11/22) and moderate (9/22) to strong (8/22) staining. In contrast, only 6 of 31 SCCs (19.4%) expressed CD117 focally, and this expression was limited to the basal layer of the tumor in four cases. CEA immunostaining highlighted the lumina of all 22 porocarcinomas; however, CEA expression was not significantly different between porocarcinomas and SCCs (100 vs. 71.0%, respectively). CA19-9 was not expressed in the lumina of 5 of 22 porocarcinomas. CONCLUSIONS: Along with CEA, CD117 immunohistochemistry could be helpful in distinguishing porocarcinomas from SCCs.
BACKGROUND: Distinguishing porocarcinoma from squamous cell carcinoma (SCC) is clinically significant but can pose a diagnostic dilemma. The present study sought to confirm the diagnostic utility of CD117 immunohistochemistry in distinguishing porocarcinoma from SCC and to examine histologic, carcinoembryonic antigen (CEA) immunohistochemical and CA19-9 immunohistochemical differences between these tumors. METHODS: Immunostaining with anti-CD117, anti-CEA and anti-CA19-9 antibodies was performed for 22 porocarcinomas and 31 SCCs. The extent of CD117, CEA and CA19-9 staining was classified as negative (<1%), rarely positive (1-4%), focally positive (5-29%) or diffusely positive (30-100%). CD117 staining intensity was semi-quantitatively graded as weak, moderate or strong. RESULTS: All (100%) porocarcinomas were positive for CD117, with mainly focal (8/22) or diffuse (11/22) and moderate (9/22) to strong (8/22) staining. In contrast, only 6 of 31 SCCs (19.4%) expressed CD117 focally, and this expression was limited to the basal layer of the tumor in four cases. CEA immunostaining highlighted the lumina of all 22 porocarcinomas; however, CEA expression was not significantly different between porocarcinomas and SCCs (100 vs. 71.0%, respectively). CA19-9 was not expressed in the lumina of 5 of 22 porocarcinomas. CONCLUSIONS: Along with CEA, CD117 immunohistochemistry could be helpful in distinguishing porocarcinomas from SCCs.
Authors: Tithi Roy; Samuel T Boateng; Sergette Banang-Mbeumi; Pankaj K Singh; Pratik Basnet; Roxane-Cherille N Chamcheu; Federico Ladu; Isabel Chauvin; Vladimir S Spiegelman; Ronald A Hill; Konstantin G Kousoulas; Bolni Marius Nagalo; Anthony L Walker; Jean Fotie; Siva Murru; Mario Sechi; Jean Christopher Chamcheu Journal: Bioorg Chem Date: 2020-12-30 Impact factor: 5.275
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