Literature DB >> 26445787

Immunostaining of Lymphocytes in Mouse Atherosclerotic Plaque.

Anton Gisterå1, Daniel F J Ketelhuth2.   

Abstract

Immunostaining can be defined as a method for identification of constituents in situ, e.g., proteins, using specific antigen-antibody interactions, visualized by color or fluorescence. In experimental research of atherosclerosis, immunostaining is a key method used for the characterization of the cellular composition in the atherosclerotic plaques. This includes the quantification of macrophages, smooth muscle cells, and lymphocytes-especially T lymphocytes. In this chapter we focus on basic protocols for characterization of lymphocytes and/or local T lymphocyte-related antigens in mouse atherosclerotic lesions.

Entities:  

Keywords:  Atherosclerosis; Immunofluorescence; Immunohistochemistry; Immunostaining; Lymphocytes; T lymphocytes

Mesh：

Substances：

Year:  2015        PMID： 26445787     DOI： 10.1007/978-1-4939-2929-0_10

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  3 in total

1.  Genetic Deficiency of Indoleamine 2,3-dioxygenase Aggravates Vascular but Not Liver Disease in a Nonalcoholic Steatohepatitis and Atherosclerosis Comorbidity Model.

Authors:  Aastha Arora; Gustavo Luis Tripodi; Ilona Kareinen; Martin Berg; Maria Josefa Forteza; Anton Gisterå; Silke Griepke; Felipe Beccaria Casagrande; Joilson O Martins; Dulcineia Saes Parra Abdalla; Jennifer Cole; Claudia Monaco; Daniel F J Ketelhuth
Journal:  Int J Mol Sci       Date:  2022-05-06       Impact factor: 6.208

2.  Activation of the Regulatory T-Cell/Indoleamine 2,3-Dioxygenase Axis Reduces Vascular Inflammation and Atherosclerosis in Hyperlipidemic Mice.

Authors:  Maria J Forteza; Konstantinos A Polyzos; Roland Baumgartner; Bianca E Suur; Marion Mussbacher; Daniel K Johansson; Andreas Hermansson; Göran K Hansson; Daniel F J Ketelhuth
Journal:  Front Immunol       Date:  2018-05-07       Impact factor: 7.561

3.  3-Hydroxyanthralinic acid metabolism controls the hepatic SREBP/lipoprotein axis, inhibits inflammasome activation in macrophages, and decreases atherosclerosis in Ldlr-/- mice.

Authors:  Martin Berg; Konstantinos A Polyzos; Hanna Agardh; Roland Baumgartner; Maria J Forteza; Ilona Kareinen; Anton Gisterå; Gerhard Bottcher; Eva Hurt-Camejo; Göran K Hansson; Daniel F J Ketelhuth
Journal:  Cardiovasc Res       Date:  2020-10-01       Impact factor: 10.787
  3 in total

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