Literature DB >> 2644298

Comparison of a single-antigen microimmunofluorescence assay and inclusion fluorescent-antibody assay for detecting chlamydial antibodies and correlation of the results with neutralizing ability.

E M Peterson1, R Oda, P Tse, C Gastaldi, S C Stone, L M de la Maza.   

Abstract

An inclusion fluorescent-antibody assay (IFA) with McCoy cells infected with Chlamydia trachomatis serovar L2 was compared with a single-antigen (L2) microimmunofluorescence (MIF) assay for the detection of antichalmydial antibodies. A total of 562 serum specimens were tested by both assays, and sera representing a range of titers were tested for their ability to neutralize the infectivity of C. trachomatis. Overall, there was poor correlation between the two assays (r2 = 0.62). With most sera the inclusion IFA was more sensitive. There was better correlation between IFA titer and ability to neutralize the five serovars tested (L2, L3, C, E, and F) than between the MIF assay and neutralization. In summary, the IFA appeared to be more sensitive than the MIF assay for detecting antibodies to C. trachomatis.

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Year:  1989        PMID： 2644298      PMCID： PMC267310          DOI： 10.1128/jcm.27.2.350-352.1989

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  8 in total

1.  Human serology in Chlamydia trachomatis infection with microimmunofluorescence.

Authors:  S P Wang; J T Grayston
Journal:  J Infect Dis       Date:  1974-10       Impact factor: 5.226

2.  Fluorescent antibody studies in chlamydial infections.

Authors:  S J Richmond; E O Caul
Journal:  J Clin Microbiol       Date:  1975-04       Impact factor: 5.948

3.  Protective role of magnesium in the neutralization by antibodies of Chlamydia trachomatis infectivity.

Authors:  E M Peterson; G M Zhong; E Carlson; L M de la Maza
Journal:  Infect Immun       Date:  1988-04       Impact factor: 3.441

4.  Simplified serological test for antibodies to Chlamydia trachomatis.

Authors:  B J Thomas; P Reeve; J D Oriel
Journal:  J Clin Microbiol       Date:  1976-07       Impact factor: 5.948

5.  Susceptibility of different serovars of Chlamydia trachomatis to inactivation by normal human serum.

Authors:  M F Osborn; A P Johnson; D Taylor-Robinson
Journal:  Genitourin Med       Date:  1985-08

6.  Modification of the microimmunofluorescence test to provide a routine serodiagnostic test for chlamydial infection.

Authors:  J D Treharne; S Darougar; B R Jones
Journal:  J Clin Pathol       Date:  1977-06       Impact factor: 3.411

7.  Single-antigen immunofluorescence test for chlamydial antibodies.

Authors:  P Saikku; J Paavonen
Journal:  J Clin Microbiol       Date:  1978-08       Impact factor: 5.948

8.  Postabortal Chlamydia trachomatis salpingitis: correlating risk with antigen-specific serological responses and with neutralization.

Authors:  R C Brunham; R Peeling; I Maclean; J McDowell; K Persson; S Osser
Journal:  J Infect Dis       Date:  1987-04       Impact factor: 5.226
  8 in total
  13 in total

1.  Immunization with a peptide corresponding to chlamydial heat shock protein 60 increases the humoral immune response in C3H mice to a peptide representing variable domain 4 of the major outer membrane protein of Chlamydia trachomatis.

Authors:  V L Motin; L M de la Maza; E M Peterson
Journal:  Clin Diagn Lab Immunol       Date:  1999-05

2.  Comparison of immunofluorescence tests for detecting antibodies to Chlamydia trachomatis.

Authors:  T Forsey; E Peterson
Journal:  J Clin Microbiol       Date:  1989-10       Impact factor: 5.948

3.  Differences in susceptibilities of the lymphogranuloma venereum and trachoma biovars of Chlamydia trachomatis to neutralization by immune sera.

Authors:  E M Peterson; M Hoshiko; B A Markoff; M W Lauermann; L M de la Maza
Journal:  Infect Immun       Date:  1990-04       Impact factor: 3.441

4.  Effect of immunoglobulin G isotype on the infectivity of Chlamydia trachomatis in a mouse model of intravaginal infection.

Authors:  E M Peterson; X Cheng; V L Motin; L M de la Maza
Journal:  Infect Immun       Date:  1997-07       Impact factor: 3.441

5.  Characterization of the humoral response induced by a peptide corresponding to variable domain IV of the major outer membrane protein of Chlamydia trachomatis serovar E.

Authors:  X Cheng; S Pal; L M de la Maza; E M Peterson
Journal:  Infect Immun       Date:  1992-08       Impact factor: 3.441

6.  Functional and structural mapping of Chlamydia trachomatis species-specific major outer membrane protein epitopes by use of neutralizing monoclonal antibodies.

Authors:  E M Peterson; X Cheng; B A Markoff; T J Fielder; L M de la Maza
Journal:  Infect Immun       Date:  1991-11       Impact factor: 3.441

7.  Characterization of the murine antibody response to peptides representing the variable domains of the major outer membrane protein of Chlamydia pneumoniae.

Authors:  E M Peterson; X Cheng; Z Qu; L M de La Maza
Journal:  Infect Immun       Date:  1996-08       Impact factor: 3.441

8.  Characterization of a neutralizing monoclonal antibody directed at variable domain I of the major outer membrane protein of Chlamydia trachomatis C-complex serovars.

Authors:  Z Qu; X Cheng; L M de la Maza; E M Peterson
Journal:  Infect Immun       Date:  1993-04       Impact factor: 3.441

9.  Protection against infertility in a BALB/c mouse salpingitis model by intranasal immunization with the mouse pneumonitis biovar of Chlamydia trachomatis.

Authors:  S Pal; T J Fielder; E M Peterson; L M de la Maza
Journal:  Infect Immun       Date:  1994-08       Impact factor: 3.441

10.  Analysis of the immune response in mice following intrauterine infection with the Chlamydia trachomatis mouse pneumonitis biovar.

Authors:  S Pal; T J Fielder; E M Peterson; L M de la Maza
Journal:  Infect Immun       Date:  1993-02       Impact factor: 3.441
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