Karina H Nakayama1,2,3, Prajakta A Joshi3,4, Edwina S Lai5, Prachi Gujar6, Lydia-M Joubert7, Bertha Chen6, Ngan F Huang1,2,3. 1. Stanford Cardiovascular Institute, Stanford University, Stanford, CA 94305, USA. 2. Department of Cardiothoracic Surgery, Stanford University, 300 Pasteur Drive, Stanford, CA 94305-5407, USA. 3. Veterans Affairs Palo Alto Health Care System, Palo Alto, CA 94304, USA. 4. Department of Biological Sciences, San Jose State University, San Jose, CA 95112, USA. 5. Department of Chemical Engineering, Stanford University, Stanford, CA 94305, USA. 6. Department of Obstetrics & Gynecology, Stanford University, Stanford, CA 94305, USA. 7. Cell Sciences Imaging Facility, Stanford University, Stanford, CA 94305, USA.
Abstract
BACKGROUND: We developed an aligned bi-layered vascular graft derived from human induced pluripotent stem cells (iPSCs) that recapitulates the cellular composition, orientation, and anti-inflammatory function of blood vessels. MATERIALS & METHODS: The luminal layer consisted of longitudinal-aligned nanofibrillar collagen containing primary endothelial cells (ECs) or iPSC-derived ECs (iPSC-ECs). The outer layer contained circumferentially oriented nanofibrillar collagen with primary smooth muscle cells (SMCs) or iPSC-derived SMCs(iPSC-SMCs). RESULTS: On the aligned scaffolds, cells organized F-actin assembly within 8º from the direction of nanofibrils. When compared to randomly-oriented scaffolds, EC-seeded aligned scaffolds had significant reduced inflammatory response, based on adhesivity to monocytes. CONCLUSION: This study highlights the importance of anisotropic scaffolds in directing cell form and function, and has therapeutic significance as physiologically relevant blood vessels.
BACKGROUND: We developed an aligned bi-layered vascular graft derived from human induced pluripotent stem cells (iPSCs) that recapitulates the cellular composition, orientation, and anti-inflammatory function of blood vessels. MATERIALS & METHODS: The luminal layer consisted of longitudinal-aligned nanofibrillar collagen containing primary endothelial cells (ECs) or iPSC-derived ECs (iPSC-ECs). The outer layer contained circumferentially oriented nanofibrillar collagen with primary smooth muscle cells (SMCs) or iPSC-derived SMCs(iPSC-SMCs). RESULTS: On the aligned scaffolds, cells organized F-actin assembly within 8º from the direction of nanofibrils. When compared to randomly-oriented scaffolds, EC-seeded aligned scaffolds had significant reduced inflammatory response, based on adhesivity to monocytes. CONCLUSION: This study highlights the importance of anisotropic scaffolds in directing cell form and function, and has therapeutic significance as physiologically relevant blood vessels.
Authors: Lucas P Neff; Bryan W Tillman; Saami K Yazdani; Masood A Machingal; James J Yoo; Shay Soker; Brian W Bernish; Randolph L Geary; George J Christ Journal: J Vasc Surg Date: 2011-02 Impact factor: 4.268
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