Literature DB >> 26437645

Molecular cloning and characterization of a Spirometra erinacei casein kinase I.

Li Na Liu1, Han Wang1, Peng Jiang1, Man Wang1, Ming Guo Xu1, Li Fu Wang1, Xin Qi1, Xi Zhang1, Jing Cui2, Zhong Quan Wang3.   

Abstract

The Spirometra erinacei casein kinase I (SeCKI) gene was cloned and expressed in Escherichia coli, and its characteristics were investigated in this study. The recombinant SeCP protein (rSeCKI) was purified. The vaccination of mice with rSeCKI induced the Th1/Th2-mixed type of immune response with Th2 predominant (high levels of IgG1). Western blotting analysis showed that rSeCP was recognized by the sera of plerocercoid-infected mice, and anti-rSeCP serum recognized the native SeCP protein of plerocercoid crude antigens. Transcription and expression of SeCP was observed at the plerocercoid and adult stages of S. erinacei. Immunolocalization identified SeCKI in the tegument and parenchymal tissues of plerocercoids and in the teguments of adults. SeCKI appeared to be essential indispensable for the S. erinacei development and survival in host, but its biological functions need to be further investigated.

Entities:  

Keywords:  Casein kinase I (CKI); Plerocercoid; Sparganum; Spirometra erinacei

Mesh：

Substances：

Year:  2015        PMID： 26437645     DOI： 10.1007/s00436-015-4758-7

Source DB:  PubMed          Journal:  Parasitol Res        ISSN： 0932-0113            Impact factor:   2.289


  30 in total

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Journal:  FEBS Lett       Date:  1969-09       Impact factor: 4.124

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3.  Cerebral sparganosis: the wandering lesion.

Authors:  Kentaro Shirakawa; Hiroshi Yamasaki; Akira Ito; Hiroaki Miyajima
Journal:  Neurology       Date:  2010-01-12       Impact factor: 9.910

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5.  Protein changes in Trichinella spiralis muscle larvae in vitro induced by bovine bile.

Authors:  Li Wang; Z Q Wang; J Cui
Journal:  Vet Parasitol       Date:  2013-02-05       Impact factor: 2.738

6.  Serodiagnosis of sparganosis by ELISA using recombinant cysteine protease of Spirometra erinaceieuropaei spargana.

Authors:  Li Na Liu; Xi Zhang; Peng Jiang; Ruo Dan Liu; Jian Zhou; Rui Zhe He; Jing Cui; Zhong Quan Wang
Journal:  Parasitol Res       Date:  2014-12-24       Impact factor: 2.289

7.  Characterisation of the relationship between Spirometra erinaceieuropaei and Diphyllobothrium species using complete cytb and cox1 genes.

Authors:  Xi Zhang; Han Wang; Jing Cui; Peng Jiang; Guo Mei Fu; Kan Zhong; Zi Fang Zhang; Zhong Quan Wang
Journal:  Infect Genet Evol       Date:  2015-07-21       Impact factor: 3.342

8.  Molecular characterization of a Spirometra mansoni antigenic polypeptide gene encoding a 28.7 kDa protein.

Authors:  Jing Cui; Tong Wei; Li Na Liu; Xi Zhang; Xin Qi; Zi Fang Zhang; Zhong Quan Wang
Journal:  Parasitol Res       Date:  2014-08-06       Impact factor: 2.289

9.  Depletion of casein kinase I leads to a NAD(P)(+)/NAD(P)H balance-dependent metabolic adaptation as determined by NMR spectroscopy-metabolomic profile in Kluyveromyces lactis.

Authors:  D Gorietti; E Zanni; C Palleschi; M Delfini; D Uccelletti; M Saliola; A Miccheli
Journal:  Biochim Biophys Acta       Date:  2013-10-18

10.  Characterization of Spirometra erinaceieuropaei Plerocercoid Cysteine Protease and Potential Application for Serodiagnosis of Sparganosis.

Authors:  Li Na Liu; Zhong Quan Wang; Xi Zhang; Peng Jiang; Xin Qi; Ruo Dan Liu; Zi Fang Zhang; Jing Cui
Journal:  PLoS Negl Trop Dis       Date:  2015-06-05
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