Literature DB >> 26435141

Asparagine deamidation reduces DNA-binding affinity of the Drosophila melanogaster Scr homeodomain.

Nichole E O'Connell1, Katherine Lelli1, Richard S Mann1, Arthur G Palmer2.   

Abstract

Spontaneous deamidation of asparagine is a non-enzymatic post-translational modification of proteins. Residue Asn 321 is the main site of deamidation of the Drosophila melanogaster Hox transcription factor Sex Combs Reduced (Scr). Formation of iso-aspartate, the major deamidation product, is detected by HNCACB triple-resonance NMR spectroscopy. The rate of deamidation is quantified by fitting the decay of Asn NH2 side-chain signals in a time-series of (15)N-(1)H HSQC NMR spectra. The deamidated form of Scr binds to specific DNA target sequences with reduced affinity as determined by an electrophoretic mobility shift assay.
Copyright © 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Asparagine deamidation; DNA binding; Dissociation constant; Hox transcription factor; NMR spectroscopy; Sex Combs Reduced

Mesh：

Substances：

Year:  2015        PMID： 26435141      PMCID： PMC4674082          DOI： 10.1016/j.febslet.2015.09.020

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  19 in total

1.  Four-dimensional NMR spectroscopy of a 723-residue protein: chemical shift assignments and secondary structure of malate synthase g.

Authors:  Vitali Tugarinov; Ranjith Muhandiram; Ayeda Ayed; Lewis E Kay
Journal:  J Am Chem Soc       Date:  2002-08-28       Impact factor: 15.419

2.  Deamidation accelerates amyloid formation and alters amylin fiber structure.

Authors:  Emily B Dunkelberger; Lauren E Buchanan; Peter Marek; Ping Cao; Daniel P Raleigh; Martin T Zanni
Journal:  J Am Chem Soc       Date:  2012-07-17       Impact factor: 15.419

3.  Age-dependent deamidation of glutamine residues in human γS crystallin: deamidation and unstructured regions.

Authors:  Michelle Yu Sung Hooi; Mark J Raftery; Roger John Willis Truscott
Journal:  Protein Sci       Date:  2012-06-11       Impact factor: 6.725

4.  Functional specificity of a Hox protein mediated by the recognition of minor groove structure.

Authors:  Rohit Joshi; Jonathan M Passner; Remo Rohs; Rinku Jain; Alona Sosinsky; Michael A Crickmore; Vinitha Jacob; Aneel K Aggarwal; Barry Honig; Richard S Mann
Journal:  Cell       Date:  2007-11-02       Impact factor: 41.582

5.  Covalent structural changes in unfolded GroES that lead to amyloid fibril formation detected by NMR: insight into intrinsically disordered proteins.

Authors:  Hisanori Iwasa; Shunsuke Meshitsuka; Kunihiro Hongo; Tomohiro Mizobata; Yasushi Kawata
Journal:  J Biol Chem       Date:  2011-04-20       Impact factor: 5.157

Review 6.  Protein misfolding and aggregation in cataract disease and prospects for prevention.

Authors:  Kate L Moreau; Jonathan A King
Journal:  Trends Mol Med       Date:  2012-04-19       Impact factor: 11.951

7.  Structure-dependent nonenzymatic deamidation of glutaminyl and asparaginyl pentapeptides.

Authors:  N E Robinson; Z W Robinson; B R Robinson; A L Robinson; J A Robinson; M L Robinson; A B Robinson
Journal:  J Pept Res       Date:  2004-05

8.  Deamidation, isomerization, and racemization at asparaginyl and aspartyl residues in peptides. Succinimide-linked reactions that contribute to protein degradation.

Authors:  T Geiger; S Clarke
Journal:  J Biol Chem       Date:  1987-01-15       Impact factor: 5.157

9.  The spatial and temporal deployment of Dfd and Scr transcripts throughout development of Drosophila.

Authors:  A Martinez-Arias; P W Ingham; M P Scott; M E Akam
Journal:  Development       Date:  1987-08       Impact factor: 6.868

10.  Control of cellular Bcl-xL levels by deamidation-regulated degradation.

Authors:  So Hee Dho; Benjamin E Deverman; Carlo Lapid; Scott R Manson; Lu Gan; Jacob J Riehm; Rajeev Aurora; Ki-Sun Kwon; Steven J Weintraub
Journal:  PLoS Biol       Date:  2013-06-25       Impact factor: 8.029
View more
  2 in total

1.  Structural and Mechanistic Studies of the Rare Myristoylation Signal of the Feline Immunodeficiency Virus.

Authors:  Janae B Brown; Holly R Summers; Lola A Brown; Jan Marchant; Paige N Canova; Colin T O'Hern; Sophia T Abbott; Constance Nyaunu; Simon Maxwell; Talayah Johnson; Morgan B Moser; Sherimay D Ablan; Hannah Carter; Eric O Freed; Michael F Summers
Journal:  J Mol Biol       Date:  2020-05-19       Impact factor: 5.469

2.  Post-translational modifications of Drosophila melanogaster HOX protein, Sex combs reduced.

Authors:  Anirban Banerjee; Anthony Percival-Smith
Journal:  PLoS One       Date:  2020-01-13       Impact factor: 3.240
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.