Jung-Hwan Kim1, Eun-Ji Jeun1, Chun-Pyo Hong1, Seong-Hoon Kim2, Min Seong Jang1, Eun-Jung Lee1, Sook Jin Moon1, Chang Ho Yun3, Sin-Hyeog Im1, Seok-Geun Jeong4, Beom-Young Park4, Kyong-Tai Kim5, Ju-Young Seoh6, Yoon-Keun Kim7, Sung-Jong Oh8, Jun-Sang Ham9, Bo-Gie Yang10, Myoung Ho Jang11. 1. Academy of Immunology and Microbiology, Institute for Basic Science, Pohang, Korea; Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, Pohang, Korea. 2. Division of Molecular and Life Science, Department of Life Sciences, Pohang University of Science and Technology, Pohang, Korea. 3. Academy of Immunology and Microbiology, Institute for Basic Science, Pohang, Korea. 4. National Institute of Animal Science, Rural Development Administration, Jeonju, Korea. 5. Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, Pohang, Korea; Division of Molecular and Life Science, Department of Life Sciences, Pohang University of Science and Technology, Pohang, Korea. 6. Department of Microbiology, Ewha Womans University Graduate School of Medicine, Seoul, Korea. 7. Department of Medicine, Ewha Womans University Graduate School of Medicine, Seoul, Korea. 8. Faculty of Biotechnology, College of Applied Life Science, Jeju National University, Jeju, Korea. 9. National Institute of Animal Science, Rural Development Administration, Jeonju, Korea. Electronic address: hamjs@korea.kr. 10. Academy of Immunology and Microbiology, Institute for Basic Science, Pohang, Korea. Electronic address: yangbg@ibs.re.kr. 11. Academy of Immunology and Microbiology, Institute for Basic Science, Pohang, Korea. Electronic address: jangmh@ibs.re.kr.
Abstract
BACKGROUND: The incidence of food allergies has increased dramatically during the last decade. Recently, probiotics have been studied for the prevention and treatment of allergic disease. OBJECTIVE: We examined whether Bifidobacterium longum KACC 91563 and Enterococcus faecalis KACC 91532 have the capacity to suppress food allergies. METHODS: B longum KACC 91563 and E faecalis KACC 91532 were administered to BALB/c wild-type mice, in which food allergy was induced by using ovalbumin and alum. Food allergy symptoms and various immune responses were assessed. RESULTS: B longum KACC 91563, but not E faecalis KACC 91532, alleviated food allergy symptoms. Extracellular vesicles of B longum KACC 91563 bound specifically to mast cells and induced apoptosis without affecting T-cell immune responses. Furthermore, injection of family 5 extracellular solute-binding protein, a main component of extracellular vesicles, into mice markedly reduced the occurrence of diarrhea in a mouse food allergy model. CONCLUSION: B longum KACC 91563 induces apoptosis of mast cells specifically and alleviates food allergy symptoms. Accordingly, B longum KACC 91563 and family 5 extracellular solute-binding protein exhibit potential as therapeutic approaches for food allergies.
BACKGROUND: The incidence of food allergies has increased dramatically during the last decade. Recently, probiotics have been studied for the prevention and treatment of allergic disease. OBJECTIVE: We examined whether Bifidobacterium longumKACC 91563 and Enterococcus faecalis KACC 91532 have the capacity to suppress food allergies. METHODS: B longumKACC 91563 and E faecalis KACC 91532 were administered to BALB/c wild-type mice, in which food allergy was induced by using ovalbumin and alum. Food allergy symptoms and various immune responses were assessed. RESULTS: B longumKACC 91563, but not E faecalis KACC 91532, alleviated food allergy symptoms. Extracellular vesicles of B longumKACC 91563 bound specifically to mast cells and induced apoptosis without affecting T-cell immune responses. Furthermore, injection of family 5 extracellular solute-binding protein, a main component of extracellular vesicles, into mice markedly reduced the occurrence of diarrhea in a mousefood allergy model. CONCLUSION: B longumKACC 91563 induces apoptosis of mast cells specifically and alleviates food allergy symptoms. Accordingly, B longumKACC 91563 and family 5 extracellular solute-binding protein exhibit potential as therapeutic approaches for food allergies.
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