| Literature DB >> 26433069 |
Dongri Jin1, Min-Ho Seo2, Bui The Huy3, Quoc-Thai Pham2, Maxwell L Conte2, Daniel Thangadurai2, Yong-Ill Lee4.
Abstract
A convenient enzymatic optical method for uric acid detection was developed based on the fluorescence quenching of ligand-capped CdTe nanoparticles by H2O2 which was generated from the enzymatic reaction of uric acid. The interactions between the CdTe nanoparticles capped with different ligands (glutathione, 3-mercaptopropionic acid, and thioglycerol) and H2O2 were investigated. The fluorescence quenching studies of GSH-capped CdTe nanoparticles demonstrated an excellent sensitivity to H2O2. The effects of uric acid, uricase and H2O2 on the fluorescence intensity of CdTe nanoparticles were also explored. The detection conditions, reaction time, pH value, incubation period and the concentration of uricase and uric acid were optimized. The detection limit of uric acid was found to be 0.10 µM and the linear range was 0.22-6 µM under the optimized experimental conditions. These results typify that CdTe nanoparticles could be used as a fluorescent probe for uric acid detection.Entities:
Keywords: Enzyme; Fluorescence quenching; Hydrogen peroxide; Nanoparticles; Uric acid
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Year: 2015 PMID： 26433069 DOI： 10.1016/j.bios.2015.09.057
Source DB: PubMed Journal: Biosens Bioelectron ISSN： 0956-5663 Impact factor: 10.618