Kazumichi Kawakubo1, Shunsuke Ohnishi2, Yutaka Hatanaka3, Kanako C Hatanaka3, Hidetaka Hosono1, Yoshimasa Kubota1, Mako Kamiya4, Masaki Kuwatani1, Hiroshi Kawakami1, Yasuteru Urano5, Naoya Sakamoto1. 1. Department of Gastroenterology and Hepatology, Hokkaido University Graduate School of Medicine, Kita15 Nishi7, Kita-ku, Sapporo, 060-8638, Japan. 2. Department of Gastroenterology and Hepatology, Hokkaido University Graduate School of Medicine, Kita15 Nishi7, Kita-ku, Sapporo, 060-8638, Japan. sonishi@pop.med.hokudai.ac.jp. 3. Department of Surgical Pathology, Hokkaido University Hospital, Sapporo, Japan. 4. Laboratory of Chemical Biology and Molecular Imaging, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan. 5. Laboratory of Chemical Biology and Molecular Imaging, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan. uranokun@m.u-tokyo.ac.jp.
Abstract
PURPOSE: Endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) is the most reliable method for the histological diagnosis of pancreatic tumors. Rapid on-site fluorescence-guided histological diagnosis was evaluated by topically applying an enzymatically activatable probe onto the EUS-FNA samples; the probe fluoresces in the presence of γ-glutamyltranspeptidase (GGT). PROCEDURES: We evaluated GGT expression in pancreatic cancer cell lines in vitro. EUS-FNA was performed in 10 pancreatic tumors. After topical application of the probe, signal intensity was measured using a fluorescence imaging system for 13 min. RESULTS: GGT was expressed in Panc-1, AsPC-1, and AR42J, but not in KP4 cells. In samples from six cases, several regions of the specimens fluoresced and contained adequate tissue for pathological diagnosis. The remaining four non-fluorescent samples contained very small amounts of carcinoma, normal epithelial cells, or no epithelial cells. The signal intensity at 5 min was 25.5 ± 7.7 and 7.7 ± 0.5 in fluorescent and non-fluorescent regions, respectively (p < 0.05). CONCLUSIONS: Application of enzymatically activatable probe onto EUS-FNA samples would be feasible for the rapid evaluation of tissues suitable for histological diagnosis.
PURPOSE: Endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) is the most reliable method for the histological diagnosis of pancreatic tumors. Rapid on-site fluorescence-guided histological diagnosis was evaluated by topically applying an enzymatically activatable probe onto the EUS-FNA samples; the probe fluoresces in the presence of γ-glutamyltranspeptidase (GGT). PROCEDURES: We evaluated GGT expression in pancreatic cancer cell lines in vitro. EUS-FNA was performed in 10 pancreatic tumors. After topical application of the probe, signal intensity was measured using a fluorescence imaging system for 13 min. RESULTS: GGT was expressed in Panc-1, AsPC-1, and AR42J, but not in KP4 cells. In samples from six cases, several regions of the specimens fluoresced and contained adequate tissue for pathological diagnosis. The remaining four non-fluorescent samples contained very small amounts of carcinoma, normal epithelial cells, or no epithelial cells. The signal intensity at 5 min was 25.5 ± 7.7 and 7.7 ± 0.5 in fluorescent and non-fluorescent regions, respectively (p < 0.05). CONCLUSIONS: Application of enzymatically activatable probe onto EUS-FNA samples would be feasible for the rapid evaluation of tissues suitable for histological diagnosis.
Authors: Robert L Schmidt; Brandon S Walker; Kirsten Howard; Lester J Layfield; Douglas G Adler Journal: Dig Dis Sci Date: 2013-07-04 Impact factor: 3.199
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