Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Construction of functional artificial minichromosomes in the fission yeast Schizosaccharomyces pombe.

Literature DB >> 2643117

Construction of functional artificial minichromosomes in the fission yeast Schizosaccharomyces pombe.

K M Hahnenberger¹, M P Baum, C M Polizzi, J Carbon, L Clarke.

Abstract

The centromere DNAs from chromosomes I and III of Schizosaccharomyces pombe have been cloned in an artificial chromosome vector in both budding and fission yeasts. In S. pombe, synthetic linear and circular minichromosomes containing an intact centromere are stable mitotically and behave as independent genetic linkage groups that segregate properly through meiosis. These experiments present a general strategy for the isolation of centromeres from other organisms.

Entities: Species

Mesh：

Substances：
DNA, Fungal

Year: 1989 PMID： 2643117 PMCID： PMC286515 DOI： 10.1073/pnas.86.2.577

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

23 in total

1. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors.

Authors: D T Burke; G F Carle; M V Olson
Journal: Science Date: 1987-05-15 Impact factor: 47.728

Review 2. The structure and function of yeast centromeres.

Authors: L Clarke; J Carbon
Journal: Annu Rev Genet Date: 1985 Impact factor: 16.830

3. Electrophoretic separations of large DNA molecules by periodic inversion of the electric field.

Authors: G F Carle; M Frank; M V Olson
Journal: Science Date: 1986-04-04 Impact factor: 47.728

4. Mitotic stability of yeast chromosomes: a colony color assay that measures nondisjunction and chromosome loss.

Authors: P Hieter; C Mann; M Snyder; R W Davis
Journal: Cell Date: 1985-02 Impact factor: 41.582

5. Genomic substitutions of centromeres in Saccharomyces cerevisiae.

Authors: L Clarke; J Carbon
Journal: Nature Date: 1983 Sep 1-7 Impact factor: 49.962

6. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.

Authors: D C Schwartz; C R Cantor
Journal: Cell Date: 1984-05 Impact factor: 41.582

7. Analysis of centromeric DNA in the fission yeast Schizosaccharomyces pombe.

Authors: L Clarke; H Amstutz; B Fishel; J Carbon
Journal: Proc Natl Acad Sci U S A Date: 1986-11 Impact factor: 11.205

8. A novel sequence common to the centromere regions of Schizosaccharomyces pombe chromosomes.

Authors: Y Nakaseko; N Kinoshita; M Yanagida
Journal: Nucleic Acids Res Date: 1987-06-25 Impact factor: 16.971

9. Isolation of a yeast centromere and construction of functional small circular chromosomes.

Authors: L Clarke; J Carbon
Journal: Nature Date: 1980-10-09 Impact factor: 49.962

10. Rearrangements of the transposable mating-type cassettes of fission yeast.

Authors: D H Beach; A J Klar
Journal: EMBO J Date: 1984-03 Impact factor: 11.598

60 in total

1. Chlamydomonas telomere sequences are A+T-rich but contain three consecutive G-C base pairs.

Authors: M E Petracek; P A Lefebvre; C D Silflow; J Berman
Journal: Proc Natl Acad Sci U S A Date: 1990-11 Impact factor: 11.205

2. Partial deletion of alpha satellite DNA associated with reduced amounts of the centromere protein CENP-B in a mitotically stable human chromosome rearrangement.

Authors: R Wevrick; W C Earnshaw; P N Howard-Peebles; H F Willard
Journal: Mol Cell Biol Date: 1990-12 Impact factor: 4.272

Construction of functional artificial minichromosomes in the fission yeast Schizosaccharomyces pombe.

1. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors.

Review 2. The structure and function of yeast centromeres.

3. Electrophoretic separations of large DNA molecules by periodic inversion of the electric field.

4. Mitotic stability of yeast chromosomes: a colony color assay that measures nondisjunction and chromosome loss.

5. Genomic substitutions of centromeres in Saccharomyces cerevisiae.

6. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.

7. Analysis of centromeric DNA in the fission yeast Schizosaccharomyces pombe.

8. A novel sequence common to the centromere regions of Schizosaccharomyces pombe chromosomes.

9. Isolation of a yeast centromere and construction of functional small circular chromosomes.

10. Rearrangements of the transposable mating-type cassettes of fission yeast.

1. Chlamydomonas telomere sequences are A+T-rich but contain three consecutive G-C base pairs.

2. Partial deletion of alpha satellite DNA associated with reduced amounts of the centromere protein CENP-B in a mitotically stable human chromosome rearrangement.

Review 3. The role of heterochromatin in centromere function.

4. Chromosome segregation in fission yeast with mutations in the tubulin folding cofactor D.

5. Introduction of large linear minichromosomes into Schizosaccharomyces pombe by an improved transformation procedure.

Review 6. Chromosome manipulation: a systematic approach toward understanding human chromosome structure and function.

Review 7. Centromere DNA dynamics: latent centromeres and neocentromere formation.

8. Functional differentiation of tbf1 orthologues in fission and budding yeasts.

9. Rapid screening of a human genomic library in yeast artificial chromosomes for single-copy sequences.

Review 10. Focus on the centre: the role of chromatin on the regulation of centromere identity and function.