Mina Kazemzadeh1, Reza Safaralizadeh1, Mohammad Ali HosseinPour Feizi1, Mohammad Hossein Somi2, Behrooz Shokoohi3. 1. Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz - Iran. 2. Liver and Gastroenterology Diseases Research Center, Tabriz University of Medical Sciences, Tabriz - Iran. 3. Department of Pathology, Tabriz University of Medical Sciences, Tabriz - Iran.
Abstract
BACKGROUND: Long non-coding RNAs (lncRNAs), a class of regulatory RNAs, play a major role in various cellular processes. Long intergenic non-coding RNAs (lincRNAs), a subclass of lncRNAs, are involved in the trans- and cis-regulation of gene expression. In the case of cis-regulation, by recruiting chromatin-modifying complexes, lincRNAs influence adjacent gene expression. METHODS: We used quantitative real-time reverse-transcription polymerase chain reaction (qRT-PCR) to evaluate the coexpression of LOC100287225, a lincRNA, and DCC, one of its adjacent genes that is often decreased in colorectal cancer, in pairs of tumor and adjacent tumor-free tissues of 30 colorectal cancer patients. RESULTS: The qRT-PCR results revealed the misregulation of these genes during tumorigenesis. Their relative expression levels were significantly lower in tumor tissues than adjacent tumor-free tissues. However, the analysis found no significant correlation between reduced expression of these genes. CONCLUSIONS: Our study demonstrated the concurrent misregulation of DCC and LOC100287225 in colorectal cancer.
BACKGROUND: Long non-coding RNAs (lncRNAs), a class of regulatory RNAs, play a major role in various cellular processes. Long intergenic non-coding RNAs (lincRNAs), a subclass of lncRNAs, are involved in the trans- and cis-regulation of gene expression. In the case of cis-regulation, by recruiting chromatin-modifying complexes, lincRNAs influence adjacent gene expression. METHODS: We used quantitative real-time reverse-transcription polymerase chain reaction (qRT-PCR) to evaluate the coexpression of LOC100287225, a lincRNA, and DCC, one of its adjacent genes that is often decreased in colorectal cancer, in pairs of tumor and adjacent tumor-free tissues of 30 colorectal cancerpatients. RESULTS: The qRT-PCR results revealed the misregulation of these genes during tumorigenesis. Their relative expression levels were significantly lower in tumor tissues than adjacent tumor-free tissues. However, the analysis found no significant correlation between reduced expression of these genes. CONCLUSIONS: Our study demonstrated the concurrent misregulation of DCC and LOC100287225 in colorectal cancer.
Authors: Orsolya Galamb; Barbara K Barták; Alexandra Kalmár; Zsófia B Nagy; Krisztina A Szigeti; Zsolt Tulassay; Peter Igaz; Béla Molnár Journal: World J Gastroenterol Date: 2019-09-14 Impact factor: 5.742
Authors: Bo Han; Yanghua He; Li Zhang; Yi Ding; Ling Lian; Chunfang Zhao; Jiuzhou Song; Ning Yang Journal: Sci Rep Date: 2017-08-31 Impact factor: 4.379