Tsui-Yin Wong1,2, Hsin-Hsin Peng1,2, Cheng-Yeu Wu1,2,3, Jan Martel1,2, David M Ojcius2,4, Fu-Yung Hsu5, John D Young1,2,6,7. 1. Laboratory of Nanomaterials, Chang Gung University, Gueishan, Taoyuan 33302, Taiwan. 2. Center for Molecular & Clinical Immunology, Chang Gung University, Gueishan, Taoyuan 33302, Taiwan. 3. Research Center of Bacterial Pathogenesis, Chang Gung University, Gueishan, Taoyuan 33302, Taiwan. 4. Department of Biomedical Sciences, University of the Pacific, Arthur Dugoni School of Dentistry, San Francisco, CA 94103, USA. 5. Department of Materials Engineering, Ming Chi University of Technology, Taishan, New Taipei City 24301, Taiwan. 6. Laboratory of Cellular Physiology & Immunology, Rockefeller University, New York, NY 10021, USA. 7. Biochemical Engineering Research Center, Ming Chi University of Technology, Taishan, New Taipei City 24301, Taiwan.
Abstract
AIMS: Mineralo-organic nanoparticles (NPs) detected in biological fluids have been described as precursors of physiological and pathological calcifications in the body. Our main objective was to examine the early stages of mineral NP formation in body fluids. MATERIALS & METHODS: A nanomaterial approach based on atomic force microscopy, dynamic light scattering, electron microscopy and spectroscopy was used. RESULTS: The mineral particles, which contain the serum proteins albumin and fetuin-A, initially precipitate in the form of round amorphous NPs that gradually grow in size, aggregate and coalesce to form crystalline mineral films similar to the structures observed in calcified human arteries. CONCLUSION: Our study reveals the early stages of particle formation and provides a platform to analyze the role(s) of mineralo-organic NPs in human tissues.
AIMS: Mineralo-organic nanoparticles (NPs) detected in biological fluids have been described as precursors of physiological and pathological calcifications in the body. Our main objective was to examine the early stages of mineral NP formation in body fluids. MATERIALS & METHODS: A nanomaterial approach based on atomic force microscopy, dynamic light scattering, electron microscopy and spectroscopy was used. RESULTS: The mineral particles, which contain the serum proteins albumin and fetuin-A, initially precipitate in the form of round amorphous NPs that gradually grow in size, aggregate and coalesce to form crystalline mineral films similar to the structures observed in calcified human arteries. CONCLUSION: Our study reveals the early stages of particle formation and provides a platform to analyze the role(s) of mineralo-organic NPs in human tissues.
Entities:
Keywords:
biological fluids; biomineralization; calcium granules; carbonate apatite; ectopic calcification; mineral biofilms; mineral particles; mineralo-organic interface
Authors: Cheng-Yeu Wu; Jan Martel; Tsui-Yin Wong; David Young; Chien-Chun Liu; Cheng-Wei Lin; John D Young Journal: Sci Rep Date: 2016-06-28 Impact factor: 4.379
Authors: Anton G Kutikhin; Elena A Velikanova; Rinat A Mukhamadiyarov; Tatiana V Glushkova; Vadim V Borisov; Vera G Matveeva; Larisa V Antonova; Dmitriy E Filip'ev; Alexey S Golovkin; Daria K Shishkova; Andrey Yu Burago; Alexey V Frolov; Viktor Yu Dolgov; Olga S Efimova; Anna N Popova; Valentina Yu Malysheva; Alexandr A Vladimirov; Sergey A Sozinov; Zinfer R Ismagilov; Dmitriy M Russakov; Alexander A Lomzov; Dmitriy V Pyshnyi; Anton K Gutakovsky; Yuriy A Zhivodkov; Evgeniy A Demidov; Sergey E Peltek; Viatcheslav F Dolganyuk; Olga O Babich; Evgeniy V Grigoriev; Elena B Brusina; Olga L Barbarash; Arseniy E Yuzhalin Journal: Sci Rep Date: 2016-06-02 Impact factor: 4.379
Authors: Tsui-Yin Wong; Cheng-Yeu Wu; Jan Martel; Cheng-Wei Lin; Fu-Yung Hsu; David M Ojcius; Paul Y Lin; John D Young Journal: Sci Rep Date: 2015-10-26 Impact factor: 4.379
Authors: Hsin-Hsin Peng; Yu-Ju Liu; David M Ojcius; Chiou-Mei Lee; Ren-Hao Chen; Pei-Rong Huang; Jan Martel; John D Young Journal: Sci Rep Date: 2017-11-30 Impact factor: 4.379
Authors: Anton G Kutikhin; Lian Feenstra; Alexander E Kostyunin; Arseniy E Yuzhalin; Jan-Luuk Hillebrands; Guido Krenning Journal: Arterioscler Thromb Vasc Biol Date: 2021-03-11 Impact factor: 8.311