| Literature DB >> 2642839 |
S P Lake1, P D Bassett, A Larkins, J Revell, K Walczak, J Chamberlain, G M Rumford, N J London, P S Veitch, P R Bell.
Abstract
A new method is described for the large-scale purification of human pancreatic islets with a discontinuous gradient of bovine serum albumin formed on an IBM 2991 cell separator. Fifteen human pancreases were processed, and after density-gradient centrifugation, a mean of 2643 islets/ml pancreatic digest were recovered with a mean purity of 63% and contained in 430 microliter mean vol. Viability of gradient-isolated islets was compared with that of non-density-gradient islets (handpicked) and showed no difference in function. This technique allows isolation of intact, viable human islets of Langerhans of sufficient purity for potential human transplantation.Entities:
Mesh：
Year: 1989 PMID： 2642839 DOI： 10.2337/diab.38.1.s143
Source DB: PubMed Journal: Diabetes ISSN： 0012-1797 Impact factor: 9.461